Team:HKU-Hong Kong/Parts

From 2011.igem.org

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(The favorite parts are selected based on the potential to be used in our Super silencer repression system)
(The favorite parts are selected based on the potential to be used in our Super silencer repression system)
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="left"|[[Team:Part:BBa_K544001|BBa_K544001 ]]
|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="left"|[[Team:Part:BBa_K544001|BBa_K544001 ]]
|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="centre"|[[Team:Part:BBa_K544002|BBa_K544002 ]]
|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="centre"|[[Team:Part:BBa_K544002|BBa_K544002 ]]
|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="right"|[[Team:Part:BBa_K544003|BBa_K544003 ]]
|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="right"|[[Team:Part:BBa_K544003|BBa_K544003 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="left"|[[Team:Part:BBa_K544012|BBa_K544012 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="centre"|[[Team:Part:BBa_K544011|BBa_K544011 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="right"|[[Team:Part:BBa_K544013|BBa_K544013 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="left"|[[Team:Part:BBa_K544014|BBa_K544014 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="centre"|[[Team:Part:BBa_K544015|BBa_K544021 ]]
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Revision as of 14:42, 5 October 2011

Sample Data Page
Sample Data Parts.png

By expressing the tetR:H-NS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the tetR binding region (tetO2). There are two tet-R binding sites as tet-R form a dimer as in the tet repressor- operator system. As the fusion proteins bound to tet-O, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved.


Parts that we have created:

(The favorite parts are selected based on the potential to be used in our Super silencer repression system)

BBa_K544001 BBa_K544002 BBa_K544003
BBa_K544012 BBa_K544011 BBa_K544013
BBa_K544014 BBa_K544021