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Team:Kyoto/Material
From 2011.igem.org
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|07/28||ΔTMD1 Forward||cgcggcagatataatggcggt | |07/28||ΔTMD1 Forward||cgcggcagatataatggcggt | ||
| + | |- | ||
| + | |08/11||ΔTMD1 Reverse||gtcatgtttttctggcatcttcatgtct | ||
| + | |- | ||
| + | |07/28||ΔglnL Forward||gctctagaggagactgctttatggcaac | ||
| + | |- | ||
| + | |07/28||ΔglnL Reverse||ggactagtaggaactatcgtcatcgactac | ||
| + | |- | ||
| + | |07/28||ΔglnG Forward||gctctagaggtgacgtttatgcaacga | ||
| + | |- | ||
| + | |07/28||ΔglnG Reverse||ggactagtacacacaagctgtgaatcactc | ||
| + | |- | ||
| + | |07/28||ΔTMD1 Reverse||gtcatgtttttctggcatcttcatgtct | ||
| + | |- | ||
| + | |07/28||ΔTMD1 Reverse||gtcatgtttttctggcatcttcatgtct | ||
|- | |- | ||
|07/28||ΔTMD1 Reverse||gtcatgtttttctggcatcttcatgtct | |07/28||ΔTMD1 Reverse||gtcatgtttttctggcatcttcatgtct | ||
|- | |- | ||
| + | |||
|08/19, 08/24||Sam7 Forward||ggttcattcgtgaccttctcgacttc | |08/19, 08/24||Sam7 Forward||ggttcattcgtgaccttctcgacttc | ||
|- | |- | ||
Revision as of 13:53, 5 October 2011
Contents |
Materials
Strain(Fruits Fly)
| Name | Genotype and/or Relevant Features | Source or Reference |
|---|---|---|
| D.melanogaster(fly) | Oregon-R-C | DGRC[http://kyotofly.kit.jp/cgi-bin/stocks/search_res_det.cgi?DB_NUM=1&DG_NUM=105669] |
Strains(E.Coli)
| Name | Genotype and/or Relevant Features | Source or Reference |
|---|---|---|
| JM109 | endA, recA1, gyrA96,thi,hsdR17,(rκ-,mκ+),relA1, supE44,λ-,Δ(lac-proAB),[F',traD36,proAB,laclqZΔM15] | Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/] |
| BW25113 | ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514 | E.coliwiki[http://ecoliwiki.net/colipedia/index.php/BW25113] |
Primers
| Date | Name | Sequence |
|---|---|---|
| 07/21 | S Forward (Same as SRRz Forward) | gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga |
| 07/21 | S Reverse | CTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc |
| 07/21 | SRRz Reverse | CTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc |
| 07/28 | ΔTMD1 Forward | cgcggcagatataatggcggt |
| 08/11 | ΔTMD1 Reverse | gtcatgtttttctggcatcttcatgtct |
| 07/28 | ΔglnL Forward | gctctagaggagactgctttatggcaac |
| 07/28 | ΔglnL Reverse | ggactagtaggaactatcgtcatcgactac |
| 07/28 | ΔglnG Forward | gctctagaggtgacgtttatgcaacga |
| 07/28 | ΔglnG Reverse | ggactagtacacacaagctgtgaatcactc |
| 07/28 | ΔTMD1 Reverse | gtcatgtttttctggcatcttcatgtct |
| 07/28 | ΔTMD1 Reverse | gtcatgtttttctggcatcttcatgtct |
| 07/28 | ΔTMD1 Reverse | gtcatgtttttctggcatcttcatgtct |
| 08/19, 08/24 | Sam7 Forward | ggttcattcgtgaccttctcgacttc |
| 08/19, 08/24 | Sam7 Reverse | aggcgataatggcgcacatc |
| 08/20 | SRRz Forward Retry | gGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg |
| 08/20 | SRRz Reverse Retry | CTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg |
| 08/23, 08/24 | Deletion of Upstream Forward | gagcaaatccccttattgggggtaagacatg |
| 08/23, 08/24 | Deletion of Upstream Reverse | ctctagaagcggccgcgaattcc |
| 09/13, 09/22 | Add J23105 Forward | CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc |
| 09/13, 09/22 | Add J23105 Reverse | AGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc |
| 10/10 | Deletion of GFP Forward (Primer1) | ccaggcatcaaataaaacgaaaggctc |
| 10/10 | Deletion of GFP-DT Forward (Primer2) | tactagtagcggccgctgc |
| 10/10 | Deletion of GFP, GFP-DT Reverse (Primer3) | ctctagtattattgatttctaccatcttctactcc |
| - | VF2 (<partinfo>G00100</partinfo>) | tgccacctgacgtctaagaa |
| - | VF (<partinfo>G00101</partinfo>) | attaccgcctttgagtgagc |
