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Team:Edinburgh/Project
From 2011.igem.org
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* If a simple test system is desired, make fusion-ready amylase? | * If a simple test system is desired, make fusion-ready amylase? | ||
| - | * Make or acquire fusion-ready pVIII gene. | + | * Make or acquire fusion-ready pVIII gene (perhaps from <partinfo>BBa_K415151</partinfo>). |
| + | ** Remember it has a leader sequence. Our final fusion will need the leader sequence followed by the gene of interest followed by pVIII proper. | ||
* Make or acquire fusion-ready pIII gene? | * Make or acquire fusion-ready pIII gene? | ||
| + | ** Remember it has a leader sequence. Our final fusion will need the leader sequence followed by the gene of interest followed by pIII proper. | ||
* Make or acquire fusion-ready cell-surface display parts (see [[Team:Berkeley Wetlab/Cell Surface Display Parts|Berkeley 2009]]). | * Make or acquire fusion-ready cell-surface display parts (see [[Team:Berkeley Wetlab/Cell Surface Display Parts|Berkeley 2009]]). | ||
Revision as of 10:56, 29 June 2011
A project description and safety proposal is supposed to be submitted by July 15.
Project abstract
This year we will investigate enzyme synergy outside the cell, by creating E. coli with different cellulase enzymes displayed on the surface, as well as (if possible) M13 phage with the same enzymes displayed on the pVIII coat protein.
Pages
Todo
- Make or acquire fusion-ready cellulases (e.g. <partinfo>BBa_K392006</partinfo>, <partinfo>BBa_K392007</partinfo>, <partinfo>BBa_K392008</partinfo>).
- Acquire M13 phage.
- If a simple test system is desired, make fusion-ready amylase?
- Make or acquire fusion-ready pVIII gene (perhaps from <partinfo>BBa_K415151</partinfo>).
- Remember it has a leader sequence. Our final fusion will need the leader sequence followed by the gene of interest followed by pVIII proper.
- Make or acquire fusion-ready pIII gene?
- Remember it has a leader sequence. Our final fusion will need the leader sequence followed by the gene of interest followed by pIII proper.
- Make or acquire fusion-ready cell-surface display parts (see Berkeley 2009).
- Fuse whatever enzymes to cell-display parts or pVIII or maybe pIII.
This was the old Navbox for Edinburgh; now it's obsolete...
- Edinburgh 2011
- Project documentation: Project - BioSandwich - Parts - Modeling - Lab Notebook - Safety - Team - Attributions
- Pages for members: Wiki Watch - Useful Links - Sequences - Primers - Practices - Official Profile (has email addresses)
- (edit this navigation box)
