Team:Tokyo-NoKoGen/photocontrol
From 2011.igem.org
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<p class="style55"><span class="style53">4-2. Aggregation</span></p> | <p class="style55"><span class="style53">4-2. Aggregation</span></p> | ||
<p class="style55"><img src="Resources/phototaxisfig10.jpeg" border=0 width=326 height=215 alt="phototaxisfig10" style="vertical-align:baseline"></p> | <p class="style55"><img src="Resources/phototaxisfig10.jpeg" border=0 width=326 height=215 alt="phototaxisfig10" style="vertical-align:baseline"></p> | ||
- | <p class="style59">This is the result of antigen43 expression (fig. | + | <p class="style59">This is the result of antigen43 expression (fig.11).</p> |
<p class="style59">The red and orange line represents growth curve of cells containing Antigen43 under PLlacO1, and the green and the blue lines represents growth curve of cells containing no Antigen43 gene. There is a significance difference between cells with and without the aggregation proteins, as the OD595 went down just went down to a third within an hour. The OD595 of the cells for control remained stable. However, we could not see the difference between the IPTG induced cells and non-induced cells. This data could suggest that the expression of Antigen43 is not repressed well enough, and is leaking even without being induced. It might also indicate that aggregation can even happen with a small expression.</p> | <p class="style59">The red and orange line represents growth curve of cells containing Antigen43 under PLlacO1, and the green and the blue lines represents growth curve of cells containing no Antigen43 gene. There is a significance difference between cells with and without the aggregation proteins, as the OD595 went down just went down to a third within an hour. The OD595 of the cells for control remained stable. However, we could not see the difference between the IPTG induced cells and non-induced cells. This data could suggest that the expression of Antigen43 is not repressed well enough, and is leaking even without being induced. It might also indicate that aggregation can even happen with a small expression.</p> | ||
<p class="style59"> </p> | <p class="style59"> </p> | ||
<p class="style55"><span class="style53">4-3. Lysis</span></p> | <p class="style55"><span class="style53">4-3. Lysis</span></p> | ||
<p class="style59"><img src="Resources/phototaxisfig11.jpeg" border=0 width=401 height=230 alt="phototaxisfig11" style="vertical-align:baseline"></p> | <p class="style59"><img src="Resources/phototaxisfig11.jpeg" border=0 width=401 height=230 alt="phototaxisfig11" style="vertical-align:baseline"></p> | ||
- | <p class="style59">This is the result of comparison of lysis gene expression with and without antiholin (fig. | + | <p class="style59">This is the result of comparison of lysis gene expression with and without antiholin (fig.12). The yellow line represents control, vector without lysis gene. The blue line shows lysis gene with antiholin and the red line shows lysis gene without antiholin. As you can see, both blue and red line show lower OD660 than the control, indicating that there might be a leakage of lysis gene expression. However system with antiholin kept a higher OD660 than the system without antiholin. Therefore, we concluded that the inducible lysis system consisting of T4 endolysin and holin, functions better with the presence of antiholn.</p> |
<p class="style59"><img src="Resources/phototaxisfig12.jpeg" border=0 width=415 height=211 alt="phototaxisfig12" style="vertical-align:baseline"></p> | <p class="style59"><img src="Resources/phototaxisfig12.jpeg" border=0 width=415 height=211 alt="phototaxisfig12" style="vertical-align:baseline"></p> | ||
- | <p class="style59">This is the result of lysis gene expression with antiholin, induced by IPTG at stationary phase. Both of these cultivation curves indicate E. coli with lysis genes with antiholin. IPTG was not added to the yellow culture, and IPTG (f.c. 3mM) was added to the blue culture. Within 2 hours after addition of IPTG, the OD660 went down by 80%, indicating that the lysis genes were successfully induced by IPTG. </p> | + | <p class="style59">This is the result of lysis gene expression with antiholin, induced by IPTG at stationary phase (fig.13). Both of these cultivation curves indicate E. coli with lysis genes with antiholin. IPTG was not added to the yellow culture, and IPTG (f.c. 3mM) was added to the blue culture. Within 2 hours after addition of IPTG, the OD660 went down by 80%, indicating that the lysis genes were successfully induced by IPTG. </p> |
<p class="style59"> </p> | <p class="style59"> </p> | ||
<p class="style59"> </p> | <p class="style59"> </p> |
Revision as of 09:57, 5 October 2011
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Tokyo-NokoGen 2011 Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology |
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