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Team:Tokyo Metropolitan/Notebook/A31
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Revision as of 09:31, 5 October 2011
Speeding Up
Member Watanabe
-Treat restriction enzyme HNS(wt) -> X,S I120260 -> Bln I for two hours.
-DNA refining Phenol/Chloroform preparation and ethanol precipitation
-Measurement of DNA concentration 0.04 ug/ml
-Ligation HNS(wt):I20260 (expect GFP) = 3:1
-Transformation Transformed DAN solution.
