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Team:OUC-China/Project/PooSb/pc
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| + | <h2>Introduction</h2> | ||
| + | <p> According to the five clear quorum sensing system we summarize in the New QS Device, we could build a promoting cycle imitating the Wu xing’s promoting cycle.</p> | ||
| + | <img class="float=left" " src="https://static.igem.org/mediawiki/2011/3/38/OUC-China.pc1.jpg"/> | ||
| + | <img class="float=right" " src="https://static.igem.org/mediawiki/2011/f/f2/OUC-China.pc.02.jpg"/> | ||
| + | <br> | ||
| + | <div class="clear"></div> | ||
| + | <h2>General mechanism</h2> | ||
| + | <p> In the promoting cycle, Every device of our five element cycle is composed by 4 basic segments: activator protein production; AHL synthetase production; protein LeuB production and reporter fluorescent protein expression.</p> | ||
| + | <img style="margin-left:15px;" src="https://static.igem.org/mediawiki/2011/6/6c/OUC-China.pc3.jpg"/> | ||
| + | <p> The first promoter is ptetR，as the E.coli chassis we used do not contains the tetR, so it have a constitutive expression, generating the activator protein, like luxR，lasR, cinR, sinR, rhiR. When the input signal, namely AHLs, meet the activator. They will bind with each other and dimerize, forming a dimmer, which will bind the cassette in the second promoter in the picture, activating the downstream genes’ expression.<br> | ||
Revision as of 07:09, 5 October 2011
Introduction
According to the five clear quorum sensing system we summarize in the New QS Device, we could build a promoting cycle imitating the Wu xing’s promoting cycle.
General mechanism
In the promoting cycle, Every device of our five element cycle is composed by 4 basic segments: activator protein production; AHL synthetase production; protein LeuB production and reporter fluorescent protein expression.
The first promoter is ptetR，as the E.coli chassis we used do not contains the tetR, so it have a constitutive expression, generating the activator protein, like luxR，lasR, cinR, sinR, rhiR. When the input signal, namely AHLs, meet the activator. They will bind with each other and dimerize, forming a dimmer, which will bind the cassette in the second promoter in the picture, activating the downstream genes’ expression.
