Team:Peking R/Notebook/ZZR

From 2011.igem.org

(Difference between revisions)

Revision as of 19:53, 4 October 2011

Template:Https://2011.igem.org/Team:Peking R/bannerhidden Template:Https://2011.igem.org/Team:Peking R/back2 Template:Https://2011.igem.org/Team:Peking R/Notebookbackground <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> 无标题文档

Zhang Zhenrun's Notebook

	I participate in designing the project and detailing the experiments. Particularly I'm in charge of constructing vio operon parts containing ribozymes so that we can enhance the product purity of the metabolic pathway.
		Download his note
	Contents:


	07.21-07.28 Construted Terminator-pBAD promoter for part assembly in the next step. 07.29-08.05 Attended iGEM 2011 China Meetup and had a few days’ holiday. 08.06-08.13 Characterize deoxyviolacein primarily by TLC (developed by dichloromethane: methanol = 6:1 (v/v)) and spectrophotometry (absorbance wavelength=570nm). Constructed Terminator(B0015)-pBAD-vioABD. 08.14-08.18 Constructed Terminator(B0015)-pBAD-vioC with scores of base pair from vioB at 5’ and scores of base pair from vioE, termed “vioCC”. Sequencing results showed that it is actually Terminator(B0015)-pBAD-vioD with scores of base pair from vioB at 5’ and scores of base pair from vioE, termed “vioDD”. Constructed Terminator(B0015)-pBAD-vioE. 08.19-08.25 Constructed Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioE & Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE, both of whose ribosomal binding sites were mutated to AGGAGGT. 08.26-08.31 Constructed Terminator(B0015)-pBAD-vioABDE. 09.01-09.08 Constructed Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE, whose ribosomal binding site is native. 09.09-09.15 Constructed Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioD. Constructed Terminator(B0015)-pBAD-vioABD- Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE, in the backbone of pSB3K3. 09.16-09.23 Acquired HPLC results of the extraction from E. coli producing proviolacein and deoxychromoviridans. Constructed Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioD-Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE & Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioD -Terminator(B0015) -pBAD-vioE. 09.24-10.04 Constructed Terminator(B0015)-pBAD-vioAB- Terminator(B0015)-pBAD- TPP ribozyme 1.20-vioD- Terminator(B0015) -pBAD-vioE. Switched the backbone of Terminator(B0015)-pBAD-vioABD- Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE to pSB1C3. Part submission. Wiki editing, part documentation.

	==click here to his page==
	==click here to return==

Notebook	>>	Personal Note	>>	Zhang Zhenrun

@@ Line 3: / Line 3: @@
 {{https://2011.igem.org/Team:Peking_R/back2}}
 {{https://2011.igem.org/Team:Peking_R/Notebookbackground}}
+<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
 <html xmlns="http://www.w3.org/1999/xhtml">
 <head>
@@ Line 13: / Line 14: @@
 	top:325px;
 	width:640px;
-	height:9452px;
+	height:650px;
 	z-index:1;
 	font-family: "Comic Sans MS", cursive;
@@ Line 19: / Line 20: @@
 	color: #000;
 	text-align: justify;
+	font-style: normal;
+	font-weight: normal;
 }
 .notbookmaintitle {
@@ Line 24: / Line 27: @@
 	font-family: "Arial Black", Gadget, sans-serif;
 	color: #666;
-	font-weight: bold;
 }
 .name {
 	font-size: 16px;
-	font-weight: bold;
 	font-family: "Comic Sans MS", cursive;
 	color: #333;
@@ Line 99: / Line 100: @@
 #apDiv3 {
 	position:absolute;
-	left:36px;
+	left:41px;
-	top:291px;
+	top:275px;
 	width:540px;
 	height:28px;
@@ Line 123: / Line 124: @@
 	text-decoration: none;
 }
-.Iam {	font-family: "Comic Sans MS", cursive;
+.clickteam #apDiv1 table tr th p {
-	color: #000;
+	font-family: Georgia, "Times New Roman", Times, serif;
+	font-style:normal;
+}
+asdf {
+	font-weight: normal;
+}
+qqq {
+	font-weight: bold;
+}
+a {
+	font-weight: bold;
+}
+a {
+	font-weight: bold;
+}
+a {
 	font-weight: bold;
+}
+th {
+	font-family: Georgia, "Times New Roman", Times, serif;
+	font-size: 14px;
+	font-style: normal;
+	font-weight: normal;
+	font-variant: normal;
+	color: #000;
+}
+.clickteam #apDiv1 table {
+	color: #000;
+}
+p {
+	color: #000;
+}
+a {
+	color: #000;
+}
+th {
+	font-family: Georgia, "Times New Roman", Times, serif;
+	font-size: 14px;
+	font-style: normal;
+	font-weight: normal;
+	font-variant: normal;
+	color: #000;
 }
 </style>
@@ Line 134: / Line 175: @@
    <table width="600" border="0" cellspacing="0" cellpadding="0">
      <tr>
-       <th height="38" colspan="5" bgcolor="#DCF8A9" class="notbookmaintitle" scope="col">Zhenrun Zhang's Notebook</th>
+       <th height="38" colspan="5" bgcolor="#FFFFFF" class="notbookmaintitle" scope="col">Zhang Zhenrun's Notebook</th>
      </tr>
      <tr>
-       <th colspan="5" bgcolor="#DCF8A9" class="notbookmaintitle" scope="col"><hr /></th>
+       <th colspan="5" bgcolor="#FFFFFF" class="notbookmaintitle" scope="col"><hr /></th>
      </tr>
      <tr>
-       <th width="16" height="94" bgcolor="#DCF8A9" scope="row"><p>&nbsp;</p>
+       <th width="16" height="94" bgcolor="#FFFFFF" scope="row"><p>&nbsp;</p>
        <p>&nbsp;</p></th>
-       <th colspan="2" bgcolor="#DCF8A9" scope="row"><span class="selfstatement3">I participate in designing the project and detailing the experiments. Particularly I'm in charge of constructing vio operon parts containing ribozymes so that we can enhance the product purity of the metabolic pathway.</span></th>
+       <th colspan="2" bgcolor="#FFFFFF" scope="row"><p class="selfstatement3">I participate in designing the project and detailing the experiments. Particularly I'm in charge of constructing vio operon parts containing ribozymes so that we can enhance the product purity of the metabolic pathway.</p></th>
-       <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Peking_R/Team/ZZR"><img src="https://static.igem.org/mediawiki/2011/3/3c/PekingR_jerry11.jpg" width="100" height="140" /></a></p></th>
+       <th width="125" rowspan="6" bgcolor="#FFFFFF" scope="row"><p>&nbsp;</p></th>
-       <th width="20" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+       <th width="20" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
      </tr>
      <tr>
-       <th rowspan="40" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+       <th rowspan="40" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
-       <th width="29" height="29" bgcolor="#DCF8A9" scope="row"><img src="https://static.igem.org/mediawiki/2011/0/0c/PekingR_PDF_download_logo.png" alt="" width="30" height="29" /></th>
+       <th width="29" height="66" bgcolor="#FFFFFF" scope="row"><img src="https://static.igem.org/mediawiki/2011/0/0c/PekingR_PDF_download_logo.png" alt="" width="30" height="29" /></th>
-       <th width="410" nowrap="nowrap" bgcolor="#DCF8A9" class="download" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook/ZZR" class="clickteam">Download his note</a></th>
+       <th width="410" nowrap="nowrap" bgcolor="#FFFFFF" class="download" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook/ZZR" class="clickteam">Download his note</a></th>
-       <th rowspan="40" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+       <th rowspan="40" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
      </tr>
      <tr>
-       <th height="23" colspan="2" bgcolor="#DCF8A9" class="name" scope="row">Contents:</th>
+       <th height="23" colspan="2" bgcolor="#FFFFFF" class="name" scope="row">Contents:</th>
      </tr>
      <tr>
-       <th height="8" colspan="2" bgcolor="#DCF8A9" scope="row"><hr /></th>
+       <th height="8" colspan="2" bgcolor="#FFFFFF" scope="row"><hr /></th>
      </tr>
      <tr>
-       <th colspan="2" bgcolor="#DCF8A9" class="July"> <a href="#July" class="clickteam">*July,2011</a></th>
      </tr>
      <tr>
-      <th colspan="2" bgcolor="#DCF8A9" class="July" scope="row"><a href="#Augest" class="clickteam">*Augest,2011</a></th>
+       <th colspan="3" align="left" bgcolor="#FFFFFF" scope="row"><p>07.21-07.28  <br />
-    </tr>
+Construted Terminator-pBAD promoter for  part assembly in the next step.</p>
-    <tr>
+        <p>07.29-08.05  <br />
-      <th colspan="3" bgcolor="#DCF8A9" class="July" scope="row"><a href="#September" class="clickteam">*September,2011</a></th>
+          Attended iGEM 2011 China Meetup and had a  few days&rsquo; holiday.</p>
-    </tr>
+         <p>08.06-08.13  <br />
-    <tr>
+          Characterize deoxyviolacein primarily by  TLC (developed by dichloromethane: methanol = 6:1 (v/v)) and spectrophotometry  (absorbance wavelength=570nm).<br />
-      <th colspan="3" bgcolor="#DCF8A9" class="July" scope="row"><a href="#October" class="clickteam">*October,2011</a></th>
+          Constructed Terminator(B0015)-pBAD-vioABD. </p>
-    </tr>
+         <p>08.14-08.18 <br />
-    <tr>
+          Constructed Terminator(B0015)-pBAD-vioC  with scores of base pair from vioB at 5&rsquo; and scores of base pair from vioE,  termed &ldquo;vioCC&rdquo;. Sequencing results showed that it is actually  Terminator(B0015)-pBAD-vioD with scores of base pair from vioB at 5&rsquo; and scores  of base pair from vioE, termed &ldquo;vioDD&rdquo;.<br />
-      <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th>
+           Constructed Terminator(B0015)-pBAD-vioE.</p>
-    </tr>
+        <p>08.19-08.25<br />
-    <tr>
+           Constructed Terminator(B0015)-pBAD-TPP  ribozyme 1.20-vioE &amp; Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE, both of  whose ribosomal binding sites were mutated to AGGAGGT. </p>
-      <th colspan="3" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+         <p>08.26-08.31<br />
-    </tr>
+           Constructed Terminator(B0015)-pBAD-vioABDE.</p>
-    <tr>
+         <p>09.01-09.08<br />
-      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">July
+          Constructed Terminator(B0015)-pBAD-TPP  ribozyme 2.5-vioE, whose ribosomal binding site is native.</p>
-      <a name="July" id="July"></a>        <hr /></th>
+         <p>09.09-09.15<br />
-    </tr>
+           Constructed Terminator(B0015)-pBAD-TPP  ribozyme 1.20-vioD. <br />
-    <tr>
+           Constructed Terminator(B0015)-pBAD-vioABD-  Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE</a>, in the backbone of  pSB3K3.</p>
-      <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
+        <p>09.16-09.23<br />
-        <tr>
+           Acquired HPLC results of the extraction  from E. coli producing proviolacein and deoxychromoviridans.<br />
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
+           Constructed Terminator(B0015)-pBAD-TPP  ribozyme 1.20-vioD-Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE &amp;  Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioD -Terminator(B0015) -pBAD-vioE.</p>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
+         <p>09.24-10.04<br />
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
+           Constructed Terminator(B0015)-pBAD-vioAB- Terminator(B0015)-pBAD-  TPP ribozyme 1.20-vioD- Terminator(B0015) -pBAD-vioE.<br />
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
+           Switched the backbone of  Terminator(B0015)-pBAD-vioABD- Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE to  pSB1C3.<br />
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
+          Part submission.<br />
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
+       Wiki editing, part documentation.</p></th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
-        </tr>
-        <tr>
-          <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
-          <td bgcolor="#DCF8A9" class="sunday"><span class="sunday">-</span></td>
-          <td bgcolor="#DCF8A9" class="sunday">-</td>
-          <td bgcolor="#DCF8A9" class="sunday">-</td>
-          <td bgcolor="#DCF8A9" class="sunday">-</td>
-          <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d701" class="clickteam">1</a></td>
-          <td bgcolor="#DCF8A9" class="sunday"><a href="#d702" class="clickteam">2</a></td>
-        </tr>
-        <tr class="clickteam">
-          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#703" class="clickteam">3</a></span></th>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d704" class="clickteam">4</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d705" class="clickteam">5</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d707" class="clickteam">7</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d708" class="clickteam">8</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d707" class="clickteam">9</a></span></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d710" class="clickteam">10</a></span></th>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d711" class="clickteam">11</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d712" class="clickteam">12</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d713" class="clickteam">13</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d714" class="clickteam">14</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d716" class="clickteam">16</a></span></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d717" class="clickteam">17</a></span></th>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d718" class="clickteam">18</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d719" class="clickteam">19</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d720" class="clickteam">20</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d721" class="clickteam">21</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d722" class="clickteam">22</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d723" class="clickteam">23</a></span></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d724" class="clickteam">24</a></span></th>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d725" class="clickteam">25</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d726" class="clickteam">26</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d727" class="clickteam">27</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d728" class="clickteam">28</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d729" class="clickteam">29</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d730" class="clickteam">30</a></span></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d731" class="clickteam">31</a></span></th>
-          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
-          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
-          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
-          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
-          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
-          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
-        </tr>
-      </table></th>
-    </tr>
-    <tr>
-      <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
-    </tr>
-    <tr>
-      <th height="16" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><a href="#top" class="clickteam">[Top]</a></th>
-    </tr>
-    <tr>
-      <th height="31" colspan="3" bgcolor="#DCF8A9" class="download" scope="row">&nbsp;</th>
-    </tr>
-    <tr>
-       <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3">7.1--7.7<a name="d701" id="d701"></a><a name="d702" id="d702"></a><a name="d703" id="d703"></a><a name="d704" id="d704"></a><a name="d705" id="d705"></a><a name="d706" id="d706"></a><a name="d707" id="d707"></a></p>
-      <p>&nbsp;</p>
-      <p>&nbsp;</p>
-      <p>&nbsp;</p>
-      <p class="selfstatement3"><span class="selfstatement3">7.8-7.14<a name="d708" id="d708"></a><a name="d709" id="d709"></a><a name="d710" id="d710"></a><a name="d711" id="d711"></a><a name="d712" id="d712"></a><a name="d713" id="d713"></a><a name="d714" id="d714"></a></span></p>
-      <p>&nbsp;</p>
-      <p class="selfstatement3"><span class="selfstatement3">7.15-7.20<a name="d715" id="d715"></a><a name="d716" id="d716"></a><a name="d717" id="d717"></a><a name="d718" id="d718"></a><a name="d719" id="d719"></a><a name="d720" id="d720"></a></span></p>
-      <p>&nbsp;</p>
-      <p>&nbsp;</p>
-      <p><span class="selfstatement3">7.21-7.28<a name="d721" id="d721"></a><a name="d722" id="d722"></a><a name="d723" id="d723"></a><a name="d724" id="d724"></a><a name="d725" id="d725"></a><a name="d726" id="d726"></a><a name="d727" id="d727"></a><a name="d728" id="d728"></a></span></p>
-      <p class="selfstatement3"> Construted  Terminator-pBAD promoter for part assembly in the next step.</p>
-      <p class="selfstatement3"><span class="selfstatement3">7.29-7.31<a name="d729" id="d729"></a><a name="d730" id="d730"></a><a name="d731" id="d731"></a></span></p>
-      <p class="selfstatement3">Attended  iGEM 2011 China Meetup</p></th>
-    </tr>
-    <tr>
-      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">Augest<a name="Augest" id="Augest"></a></th>
-    </tr>
-    <tr>
-      <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th>
-    </tr>
-    <tr>
-      <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
-        <tr>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
-        </tr>
-        <tr>
-          <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
-          <td bgcolor="#DCF8A9" class="sunday"><a href="#d801" class="clickteam">1</a></td>
-          <td bgcolor="#DCF8A9" class="sunday"><a href="#d802" class="clickteam">2</a></td>
-          <td bgcolor="#DCF8A9" class="sunday"><a href="#d803" class="clickteam">3</a></td>
-          <td bgcolor="#DCF8A9" class="sunday"><a href="#d804" class="clickteam">4</a></td>
-          <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d805" class="clickteam">5</a></td>
-          <td bgcolor="#DCF8A9" class="sunday"><a href="#d806" class="clickteam">6</a></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d807" class="clickteam" >7</a></th>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d808" class="clickteam">8</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d809" class="clickteam">9</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d810" class="clickteam">10</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d811" class="clickteam">11</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d812" class="clickteam">12</a></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d813" class="clickteam">13</a></span></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d814"class="clickteam" >14</a></span></th>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d815" class="clickteam">15</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d816" class="clickteam">16</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d817"class="clickteam">17</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d818"class="clickteam">18</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d819"class="clickteam">19</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d820"class="clickteam">20</a></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d821"class="clickteam">21</a></th>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d822"class="clickteam">22</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d823"class="clickteam">23</a></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d824"class="clickteam">24</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d825"class="clickteam">25</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d826"class="clickteam">26</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d827"class="clickteam">27</a></span></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d828"class="clickteam">28</a></span></th>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d829"class="clickteam">29</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d830"class="clickteam">30</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d831"class="clickteam">31</a></td>
-          <td align="center" bgcolor="#DCF8A9">-</td>
-          <td align="center" bgcolor="#DCF8A9">-</td>
-          <td align="center" bgcolor="#DCF8A9">-</td>
-        </tr>
-      </table></th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><hr /></th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><span class="download"><a href="#top" class="clickteam">[Top]</a></span></th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3"><span class="selfstatement3">8.1-8.5<a name="d801" id="d801"></a><a name="d802" id="d802"></a><a name="d803" id="d803"></a><a name="d804" id="d804"></a></span><a name="d805" id="d805"></a></p>
-         <p class="selfstatement3">had a few days’ holiday.</p>
-<p class="selfstatement3"><span class="selfstatement3">8.6-8.13<a name="d806" id="d806"></a><a name="d807" id="d807"></a><a name="d808" id="d808"></a><a name="d809" id="d809"></a><a name="d810" id="d810"></a><a name="d811" id="d811"></a></span><a name="d812" id="d812"></a><a name="d813" id="d813"></a></p>
-<p class="selfstatement3">Tested violacein characterization by TLC  (developed by dichloromethane: methanol = 6:1 (v/v)) and spectrophotometry  (absorbance wavelength=570nm).</p>
-<p class="selfstatement3">&nbsp;</p>
-         <p class="selfstatement3"><span class="selfstatement3">8.14-8.18<a name="d814" id="d814"></a><a name="d815" id="d815"></a><a name="d816" id="d816"></a><a name="d817" id="d817"></a><a name="d818" id="d818"></a></span></p>
-        <p class="selfstatement3"><span class="selfstatement3">8.19-8.25<a name="d819" id="d819"></a><a name="d820" id="d820"></a><a name="d821" id="d821"></a><a name="d822" id="d822"></a><a name="d823" id="d823"></a><a name="d824" id="d824"></a><a name="d825" id="d825"></a></span></p>
-        <p class="selfstatement3"><span class="selfstatement3">8.26-8.31<a name="d826" id="d826"></a><a name="d827" id="d827"></a><a name="d828" id="d828"></a><a name="d829" id="d829"></a><a name="d830" id="d830"></a><a name="d831" id="d831"></a></span></p>
-      <p>&nbsp;</p>
-      <p>&nbsp;</p>
-      <p>&nbsp;</p>
-      <p>&nbsp;</p>
-      <p>&nbsp;</p>
-      <p>&nbsp;</p></th>
-    </tr>
-    <tr>
-      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">September<a name="September" id="September"></a></th>
-    </tr>
-    <tr>
-      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row"><hr />
-</th>
-    </tr>
-    <tr>
-      <th height="71" colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
-        <tr>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
-           <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
-           <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
-        </tr>
-        <tr>
-          <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
-          <td bgcolor="#DCF8A9" class="sunday">-</td>
-          <td bgcolor="#DCF8A9" class="sunday">-</td>
-          <td bgcolor="#DCF8A9" class="sunday">-</td>
-          <td bgcolor="#DCF8A9" class="sunday"><a href="#d901"class="clickteam">1</a></td>
-          <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d902"class="clickteam">2</a></td>
-          <td bgcolor="#DCF8A9" class="sunday"><a href="#d903"class="clickteam">3</a></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d904"class="clickteam">4</a></th>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d905"class="clickteam">5</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d906"class="clickteam">6</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d907"class="clickteam">7</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d908"class="clickteam">8</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d909"class="clickteam">9</a></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d910"class="clickteam">10</a></span></td>
-         </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d911"class="clickteam">11</a></span></th>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d912"class="clickteam">12</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d913"class="clickteam">13</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d914"class="clickteam">14</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d915"class="clickteam">15</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d916"class="clickteam">16</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d917"class="clickteam">17</a></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d918"class="clickteam">18</a></th>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d919"class="clickteam">19</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d920"class="clickteam">20</a></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d921"class="clickteam">21</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d922"class="clickteam">22</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d923"class="clickteam">23</a></span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d924"class="clickteam">24</a></span></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d925"class="clickteam">25</a></span></th>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d926"class="clickteam">26</a></span></td>
-           <td align="center" bgcolor="#DCF8A9"><a href="#d927"class="clickteam">27</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d928"class="clickteam">28</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d929"class="clickteam">29</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d930"class="clickteam">30</a></td>
-          <td align="center" bgcolor="#DCF8A9">-</td>
-        </tr>
-      </table></th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><hr /></th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><span class="download"><a href="#top" class="clickteam">[Top]</a></span></th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3"><span class="selfstatement3">9.1-9.8<a name="d901" id="d901"></a><a name="d902" id="d902"></a><a name="d903" id="d903"></a><a name="d904" id="d904"></a><a name="d905" id="d905"></a><a name="d906" id="d906"></a><a name="d907" id="d907"></a><a name="d908" id="d908"></a></span></p>
-         <p class="selfstatement3"><span class="selfstatement3">9.9-9.15<a name="d909" id="d909"></a><a name="d910" id="d910"></a><a name="d911" id="d911"></a><a name="d912" id="d912"></a><a name="d913" id="d913"></a><a name="d914" id="d914"></a><a name="d915" id="d915"></a></span></p>
-        <p class="selfstatement3"><span class="selfstatement3">9.16-9.22<a name="d916" id="d916"></a><a name="d917" id="d917"></a><a name="d918" id="d918"></a><a name="d919" id="d919"></a><a name="d920" id="d920"></a><a name="d921" id="d921"></a><a name="d922" id="d922"></a></span></p>
-         <p class="selfstatement3"><span class="selfstatement3">9.23-9.30<a name="d923" id="d923"></a><a name="d924" id="d924"></a><a name="d925" id="d925"></a><a name="d926" id="d926"></a><a name="d927" id="d927"></a><a name="d928" id="d928"></a><a name="d929" id="d929"></a><a name="d930" id="d930"></a></span></p>
-      <p>&nbsp;</p>
-      <p>&nbsp;</p>
-      <p class="selfstatement3">&nbsp;</p>
-      <p>&nbsp;</p></th>
-    </tr>
-    <tr>
-      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">October<a name="October" id="October"></a></th>
-    </tr>
-    <tr>
-      <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th>
-    </tr>
-    <tr>
-      <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
-        <tr>
-           <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
-           <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
-           <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
-          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
-        </tr>
-        <tr>
-           <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
-          <td bgcolor="#DCF8A9" class="sunday">-</td>
-          <td bgcolor="#DCF8A9" class="sunday">-</td>
-          <td bgcolor="#DCF8A9" class="sunday">-</td>
-          <td bgcolor="#DCF8A9" class="sunday">-</td>
-          <td valign="middle" bgcolor="#DCF8A9" class="sunday">-</td>
-          <td bgcolor="#DCF8A9" class="sunday"><a href="#d1001" class="clickteam"><span class="clickteam">1</span></a></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d1002" class="clickteam">2</a></th>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d1003" class="clickteam">3</a></td>
-          <td align="center" bgcolor="#DCF8A9"><a href="#d1004" class="clickteam">4</a></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">5</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">6</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">7</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">8</span></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" ><span class="sunday">9</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">10</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">11</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">12</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">13</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">14</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">15</span></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" ><span class="sunday">16</span></th>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">17</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">18</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">19</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">20</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">21</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">22</span></td>
-         </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" ><span class="sunday">23</span></th>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">24</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">25</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">26</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">27</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">28</span></td>
-          <td align="center" bgcolor="#DCF8A9"><span class="sunday">29</span></td>
-        </tr>
-        <tr>
-          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday">30</span></th>
-          <td align="center" bgcolor="#DCF8A9" class="sunday">31</td>
-          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
-           <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
-          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
-          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
-           <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
-        </tr>
-      </table></th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><hr /></th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><span class="download"><a href="#top" class="clickteam">[Top]</a></span></th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
-    </tr>
-    <tr>
-      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p><span class="selfstatement3"><span class="selfstatement3">10.1-10.4</span><a name="d1001" id="d1001"></a><a name="d1002" id="d1002"></a><a name="d1003" id="d1003"></a><a name="d1004" id="d1004"></a></span></p>
-      <p>&nbsp;</p>
-       <p>&nbsp;</p></th>
      </tr>
      <tr>
-       <th colspan="3" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+       <th colspan="3" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
      </tr>
      <tr>
-       <th colspan="3" bgcolor="#DCF8A9" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Team/ZZR" class="clickteam">==click here to his page==</a></th>
+       <th colspan="3" bgcolor="#FFFFFF" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Team/LYP" class="clickteam">==click here to his page==</a></th>
      </tr>
      <tr>
-       <th colspan="3" bgcolor="#DCF8A9" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook">==click here to return==</a></th>
+       <th colspan="3" bgcolor="#FFFFFF" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook">==click here to return==</a></th>
      </tr>
      <tr>
-       <th colspan="3" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+       <th colspan="3" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
      </tr>
    </table>
@@ Line 552: / Line 252: @@
        <th width="146" class="clickteam" scope="col"><span class="clickteam"><a href="https://2011.igem.org/Team:Peking_R/Notebook" class="clickteam">Personal Note</a></span></th>
        <th width="35" class="clickteam" scope="col">&gt;&gt;</th>
-       <th width="252" align="left" class="clickteam" scope="col"><a href="https://2011.igem.org/Team:Peking_R/Notebook/ZZR" class="clickteam">Zhenrun Zhang</a><a name="topjuly" id="topjuly"></a><a name="topaugest" id="topaugest"></a><a name="topseptember" id="topseptember"></a><a name="topoctober" id="topoctober"></a></th>
+       <th width="252" align="left" class="clickteam" scope="col"><a href="https://2011.igem.org/Team:Peking_R/Notebook/LYP" class="clickteam">Zhang Zhenrun</a><a name="topjuly" id="topjuly"></a><a name="topaugest" id="topaugest"></a><a name="topseptember" id="topseptember"></a><a name="topoctober" id="topoctober"></a></th>
      </tr>
    </table>