Team:HKU-Hong Kong/Lab Diaries
From 2011.igem.org
(Difference between revisions)
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<LI>pSB1C3-HNS | <LI>pSB1C3-HNS | ||
<LI>Electrophoresis was carried out to confirm the success of extraction) (except J23116RH) | <LI>Electrophoresis was carried out to confirm the success of extraction) (except J23116RH) | ||
+ | <div ALIGN=CENTER> | ||
+ | {| style="width:254px;background:#99EE63;text-align:center;font-family: georgia, helvetica, arial, sans-serif;color:#000000;margin- top:5px;padding: 2px;" cellspacing="5"; | ||
+ | |- | ||
+ | |[[Image:Week_11.jpg|250px]] | ||
+ | |- | ||
+ | |Confirmation of successful extraction | ||
+ | |} | ||
+ | </div> | ||
+ | <div ALIGN=CENTER> | ||
+ | {| style="width:254px;background:#99EE63;text-align:center;font-family: georgia, helvetica, arial, sans-serif;color:#000000;margin- top:5px;padding: 2px;" cellspacing="5"; | ||
+ | |- | ||
+ | |[[Image:Week_11_(2).jpg|250px]] | ||
+ | |- | ||
+ | |Confirmation of successful extraction | ||
+ | |} | ||
+ | </div> | ||
</OL> | </OL> | ||
<LI>Purification of NdeI digested pET28a | <LI>Purification of NdeI digested pET28a |
Revision as of 18:52, 4 October 2011
Lab Diaries | |
Week 1
Transformation of reporter DNA (pEGFP-loxp-km-loxp) into DH10B (non-virulent strain E. coli) with antibiotic resistance (Chloramphenicol – Cm) | Lab Diaries |
Week 2
| Lab Diaries |
Week 3
| Lab Diaries |
Week 4-5
tetO2 – 0 and tetO2 – 4
| Lab Diaries |
Week 6
Overlap PCR was carried out to produce fusion protein gene
| Lab Diaries |
Week 7
Overlap PCR was carried out to produce fusion protein gene tetR::HNS (2-90)
| Lab Diaries |
Week 8
| Lab Diaries |
Week 9
| Lab Diaries |
Week 10
| Lab Diaries |
Week 11
| Lab Diaries |
Week 12
| Lab Diaries |
Week 13
| Lab Diaries |
Week 14
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