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Team:OUC-China/Result/aadb
From 2011.igem.org
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<p> We can preliminarily screen No.1—No.6 regions of HB101 defective bacteria. The colonies of HB101 and BL21 are both small and almost transparent. We suspect it’s because medium’s formula are not so reasonable. We prepare to do a gradient of Thi’s concentration and use liquid mediume to replace solid medium to detect.</p> | <p> We can preliminarily screen No.1—No.6 regions of HB101 defective bacteria. The colonies of HB101 and BL21 are both small and almost transparent. We suspect it’s because medium’s formula are not so reasonable. We prepare to do a gradient of Thi’s concentration and use liquid mediume to replace solid medium to detect.</p> | ||
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Revision as of 17:28, 4 October 2011
The experiment of screening defective bacteria
Step-I—Screen by streak cultivation
We get 10 single colonies of HB101 by streaking. And they’re labeled for No.1—No.10 and separately streak cultured. So on the square plates, we get ten parts which are separately culturing ten bacteria colonies. Then we prepare essential culture medium and complementary culture medium to culture them and use BL21 as the contrast to screen the defective bacteria. HB101 can’t synthetize Proline and thiamine. So we have to add these two elements.
Results in theory:
Real esults:
We can preliminarily screen No.1—No.6 regions of HB101 defective bacteria. The colonies of HB101 and BL21 are both small and almost transparent. We suspect it’s because medium’s formula are not so reasonable. We prepare to do a gradient of Thi’s concentration and use liquid mediume to replace solid medium to detect.
