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Team:OUC-China/Result/aadb
From 2011.igem.org
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<h2>Step-I—Screen by streak cultivation</h2> | <h2>Step-I—Screen by streak cultivation</h2> | ||
<img style="margin-left:20px;" src="https://static.igem.org/mediawiki/2011/4/4c/OUC-China.Step-I.jpg"/> | <img style="margin-left:20px;" src="https://static.igem.org/mediawiki/2011/4/4c/OUC-China.Step-I.jpg"/> | ||
| - | <p> We get 10 single colonies of HB101 by streaking. And they’re labeled for No.1—No.10 and separately streak cultured. So on the square plates, we get ten parts which are separately culturing ten bacteria colonies. Then we prepare essential culture medium and complementary culture medium to culture them and use BL21 as the contrast to screen the defective bacteria. HB101 can’t synthetize Proline and thiamine. So we have to add these two elements.< | + | <p> We get 10 single colonies of HB101 by streaking. And they’re labeled for No.1—No.10 and separately streak cultured. So on the square plates, we get ten parts which are separately culturing ten bacteria colonies. Then we prepare essential culture medium and complementary culture medium to culture them and use BL21 as the contrast to screen the defective bacteria. HB101 can’t synthetize Proline and thiamine. So we have to add these two elements.</p> |
<b>Results in theory:</b> | <b>Results in theory:</b> | ||
| + | <br> | ||
| + | <img style="margin-left:200px;" src="https://static.igem.org/mediawiki/2011/6/64/OUC-China.sp2.JPG"/> | ||
Revision as of 17:17, 4 October 2011
The experiment of screening defective bacteria
Step-I—Screen by streak cultivation
We get 10 single colonies of HB101 by streaking. And they’re labeled for No.1—No.10 and separately streak cultured. So on the square plates, we get ten parts which are separately culturing ten bacteria colonies. Then we prepare essential culture medium and complementary culture medium to culture them and use BL21 as the contrast to screen the defective bacteria. HB101 can’t synthetize Proline and thiamine. So we have to add these two elements.
Results in theory:
