Team:Fudan-Shanghai/Project

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<td colspan=2><h1><img src="https://static.igem.org/mediawiki/2011/e/ee/Fudan_Etree.jpg  " alt="E.tree" width=100 align=center hspace=20><b> Part II: Neon Lights </b></h1></td>
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<td  bgcolor= #FFE6D9 width=600  ><h1>Detail:</h1><font size=3>
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In the project of neon lights, we intended to have E.coli engineered for emitting one color of fluorescence during exponential phase and another color during stationary phase, so that it is convenient to identify the phase and may also be utilized for other purposes. To achieve this goal, we chose BBa_J45992, a stationary phase promoter, and BBa_J45996, only active during exponential phase. Then we tried to produce different luciferases under the control of the promoters. Parts of various luciferases have been obtained by the team of Cambridge in 2010, for example, BBa_K325210, BBa_K325229, etc. System of degradation, such as ClpXP, can be used to optimize the project by degrading the previous luciferase when the latter is being produced, to make the latter color purer and the process of color changing faster. In addition, the fluctuation of the lusiferase activity due to the growth should be taken into consideration.
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<td bgcolor=#E2C2DE align=center><a href="https://static.igem.org/mediawiki/2011/d/d1/Neonlights.JPG"><img src="https://static.igem.org/mediawiki/2011/d/d1/Neonlights.JPG" width=300></a></td>
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<td colspan=2><h1><img src="https://static.igem.org/mediawiki/2011/e/ee/Fudan_Etree.jpg  " alt="E.tree" width=100  align=center hspace=20><b> Part III: Dinner Service </b></h1></td>
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<td rowspan=2 bgcolor=#ECECFF width=400  ><h1>Detail:</h1><font size=3>
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The hungry customer gives signal "luxI",which means order the dinner. The "chef" containing luxr which can sense the "order" signal  starts the generation of "dinner"-----luxpR. The dinner service is companied with yellow fluorescent,given by the expression of YFP by the "chef". When the "customer" is satisfied with the dinner, it  gives the signal of RFP and expresses aiiA to inhibite the generation of luxpR and stop serving the "dinner".
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<td bgcolor=#FFFFCE align=center><a href="https://static.igem.org/mediawiki/2011/a/a9/E.tree_detail.JPG"><img src="" height=300 alt="dinner service"></a></td>
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== '''Overall project''' ==
 
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What is the first thing that comes to your mind when you see these three words: tree, neon lights and dinner service?  Christmas. Well, exactly, and that is what our project is all about.
 
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Our synthetic biology project will modify several E.coli to perform different jobs:
 
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(1)'''Part I: E.tree'''
 
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The tree includes the leaf part and the trunk part. The “leaves” will change color according to the nutrients in the “soil”: if the soil is rich in nitrates, the “leaves” are green and healthy; otherwise, the leaves will turn yellow.
 
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(2) '''Part II: neon lights'''
 
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Each engineered E.coli can emit one light at first (such as red); after a while, the red light fades and another light is emitted. The different combination of such E.coli could therefore achieve the effect of neon lights.
 
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(3)'''Part III: dinner service'''
 
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The genetically modified bacteria involve a certain self-feedback system. When the “customer” is starving, it orders dinner from the “chef”; and the chef serves meals. While the “customer” is full, it releases a signal molecule and tells the “chef” that no more food is wanted, so the “chef” stops cooking.
 

Revision as of 15:23, 4 October 2011


2011 iGEM Fudan-Shanghai Template

2011 iGEM Fudan_Project

E.tree Part I: E.Tree

Detail:

The project “E.tree” is consisted of two genetically modified E.coli: E. trunk and E.leaf.

E.trunk detects nitrates in the medium; if KNO3 is present, the first circuit is activated, RhlI produces the AHL (C4-homoserine lactone) and TetR is synthesized to block the second pathway. If the medium lacks KNO3, the first pathway is off while the second pathway is activated and LasI produces an AHL autoinducer (3OC12-homoserine lactone).

E.leaf changes color corresponding to the signal molecules released by E.trunk. RhlR synthesized in E.leaf binds to the RhlI-directed signal, and the complex then interact with the pRhl promoter and yellow pigment is produced. This circuit also encodes RhlI, which multiple the circuit, so that all E.leaf would turn yellow. Similarly, if E.trunk releases 3OC12-homoserine lactone, which is encodes by LasI, all “leaves” would turn green.


E.tree Part II: Neon Lights

Detail:

In the project of neon lights, we intended to have E.coli engineered for emitting one color of fluorescence during exponential phase and another color during stationary phase, so that it is convenient to identify the phase and may also be utilized for other purposes. To achieve this goal, we chose BBa_J45992, a stationary phase promoter, and BBa_J45996, only active during exponential phase. Then we tried to produce different luciferases under the control of the promoters. Parts of various luciferases have been obtained by the team of Cambridge in 2010, for example, BBa_K325210, BBa_K325229, etc. System of degradation, such as ClpXP, can be used to optimize the project by degrading the previous luciferase when the latter is being produced, to make the latter color purer and the process of color changing faster. In addition, the fluctuation of the lusiferase activity due to the growth should be taken into consideration.

E.tree Part III: Dinner Service

Detail:

The hungry customer gives signal "luxI",which means order the dinner. The "chef" containing luxr which can sense the "order" signal starts the generation of "dinner"-----luxpR. The dinner service is companied with yellow fluorescent,given by the expression of YFP by the "chef". When the "customer" is satisfied with the dinner, it gives the signal of RFP and expresses aiiA to inhibite the generation of luxpR and stop serving the "dinner". 		dinner service

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