Team:HKUST-Hong Kong

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<b>Hello!</b><br>
 
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Welcome to <b>HKUST</b> Team Wiki Page!<br><br>
 
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We are the 2011 iGEM team from the <b><i>Hong Kong University of <br>Science and Technology</i> (HKUST)</b>.
 
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This is the fourth year for HKUST to<br> participate in this international synthetic biology competition.<br><br>
 
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Thanks to our experienced instructors, considerate advisors and <br>every team member from HKUST iGEM 2011 family, we enjoyed<br> a fantastic summer working cooperatively and effectively towards <br>our goal. <br><br>
 
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You may visit our <a href=gallery.html target=_top><font color=white><u>Gallery</u></font></a> to see how's our team was doing the lab.
 
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<span style="line-height:1; font-weight:600">Experiments and Results</span><br>
 
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<a href="asm.html"  target=_top>Strain construction</a> |
 
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<a href="acknowledgement.html" target=_top>Acknowledgements</a><br>
 
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<BR><BR>Human Practice</font></b></p>
 
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<b><u>Project Abstract</u></b></font></p><br>
 
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<font color=#CDD2C2>It has often been assumed that when an antibiotic is introduced to a bacterial community, only cells that carry resistance genes will survive and proliferate. However, recent findings have suggested that communities with a mixture of highly resistant (HR) and less resistant (LR) individuals are able to survive through ‘charity’ by HR individuals, which support LR individuals through indole signalling.</p><br>
 
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<p>Our team aims to interfere with this signalling through introducing a disruptor <i>E. coli</i> into the bacterial community. This new strain will be able to degrade indole using a mutated toluene-4-monooxygenase (T4MO).  We hypothesize that LR cells in the community deprived of indole will undergo eliminated at lower antibiotic concentrations. If this demonstration is successful, indole degradation might prove to be a possible strategy in boosting antibiotics effectiveness in medical practice against bacteria that rely on such signalling.</p><br>
 
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<p>Along the way, we will also create a new strain of <i>E. coli</i> that utilizes an essential gene (<i>nadE</i>) as the selection marker for transformation, allowing antibiotics-free transformation and plasmid maintenance for regular laboratory manipulation. This new transformation method can be adopted for future iGEM teams, reducing their use of antibiotics without increasing the complexity of the transformation protocol.</p><br>
 
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<a href=https://2011.igem.org/Team:HKUST-Hong_Kong><font face="Verdana, Arial, Helvetica, sans-serif" size="4" color="#FFE1E1" font color=white><span style="font-weight:700">Home</span></font></a></font></b></p>
 
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<p align="center" valign="baseline"><b> <font face="Verdana, Arial, Helvetica, sans-serif" size="3" color="green">
 
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Our Project</font></b></p>
 
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<p align="left"><font size="1" face="Verdana, Arial, Helvetica, sans-serif" color="green">
 
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<a href=team.html><font color=green>
 
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<a href="overview.html" target=_top>Overview</a> |
 
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<a href="data.html" target=_top>Data Page</a><br>
 
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<span style="line-height:1; font-weight:600">Experiments and Results</span><br>
 
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<a href="asm.html"  target=_top>Strain construction</a> |
 
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<a href="mic.html"  target=_top>Culture tests</a> |
 
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<a href="modeling.html"  target=_top>Modeling</a><br>
 
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<span style="line-height:1; font-weight:600">Miscellaneous</span><br>
 
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<a href="future.html" target=_top>Future Plans</a> |
 
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iGEM Resources</font></b></p>
 
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<p align="left"><font face="Verdana, Arial, Helvetica, sans-serif" size="1" color="#FFFFFF">
 
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<a href="acknowledgement.html" target=_top>Acknowledgements</a><br>
 
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<span style="line-height:0.7; font-weight:600">The Team</span><br>
 
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<a href="team.html" target=_top>iGEM Member List</a> |
 
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<span style="line-height:0.7; font-weight:600">Achievements</span><br>
 
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<a href="medal.html" target=_top>Medal Requirements</a> |
 
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<a href="biosafety.html" target=_top>BioSafety</a><br>
 
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<span style="line-height:0.7; font-weight:600">Biobricks</span><br>
 
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<a href="characterization.html" target=_top>Master List & Characterization Data</a>
 
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Human Practice</font></b></p>
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Revision as of 13:20, 4 October 2011


Retrieved from "http://2011.igem.org/Team:HKUST-Hong_Kong"