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Team:HKUST-Hong Kong/content.html
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| - | <b>Hello!</b | + | <b>Hello!</b><br> |
| - | Welcome to HKUST Team Wiki Page!<br><br> | + | Welcome to <b>HKUST</b> Team Wiki Page!<br><br> |
| - | We are the 2011 iGEM team from the <b><i>Hong Kong University of Science and Technology</i> (HKUST)</b>. This is the fourth year for HKUST to participate in this international synthetic biology competition.<br><br> | + | We are the 2011 iGEM team from the <b><i>Hong Kong University<br> of Science and Technology</i> (HKUST)</b>. |
| + | This is the fourth year<br> for HKUST to participate in this international synthetic<br> biology competition.<br><br> | ||
On this Wiki page, we are going to ... <br><br> | On this Wiki page, we are going to ... <br><br> | ||
| - | Thanks to our experienced instructors, considerate advisors and every team member from HKUST iGEM 2011 family, we enjoyed a fantastic summer working cooperatively and effectively towards our goal. <br><br> | + | Thanks to our experienced instructors, considerate advisors and <br>every team member from HKUST iGEM 2011 family, we enjoyed<br> a fantastic summer working cooperatively and effectively towards <br>our goal. <br><br> |
| - | Let’s now enjoy our Wiki page and see | + | Let’s now enjoy our Wiki page and see |
Revision as of 12:53, 4 October 2011
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Overview |
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Project Abstract It has often been assumed that when an antibiotic is introduced to a bacterial community, only cells that carry resistance genes will survive and proliferate. However, recent findings have suggested that communities with a mixture of highly resistant (HR) and less resistant (LR) individuals are able to survive through ‘charity’ by HR individuals, which support LR individuals through indole signalling. Our team aims to interfere with this signalling through introducing a disruptor E. coli into the bacterial community. This new strain will be able to degrade indole using a mutated toluene-4-monooxygenase (T4MO). We hypothesize that LR cells in the community deprived of indole will undergo eliminated at lower antibiotic concentrations. If this demonstration is successful, indole degradation might prove to be a possible strategy in boosting antibiotics effectiveness in medical practice against bacteria that rely on such signalling. Along the way, we will also create a new strain of E. coli that utilizes an essential gene (nadE) as the selection marker for transformation, allowing antibiotics-free transformation and plasmid maintenance for regular laboratory manipulation. This new transformation method can be adopted for future iGEM teams, reducing their use of antibiotics without increasing the complexity of the transformation protocol. |
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