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Team:NCTU Formosa/protocol G
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<body> | <body> | ||
| - | + | ||
| + | <div id="banner"><a href = "https://2011.igem.org/Team:NCTU_Formosa"><img src = "https://static.igem.org/mediawiki/2011/8/8c/Banner010.jpg" height="236" width="944"/></a></div> | ||
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<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/members">Members</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/members">Members</a></li> | ||
| - | <li><a href=" | + | <li><a href="http://www.nctu.edu.tw/english/index.php">School</a></li> |
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/gallery">Gallery</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/gallery">Gallery</a></li> | ||
</ul> | </ul> | ||
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<li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_design">Design</a></li> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_data">Data</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_data">Data</a></li> | ||
| - | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_discussion"> | + | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_discussion">Modeling</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<li><a href="https://2011.igem.org/Team:NCTU_Formosa/CI_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/CI_design">Design</a></li> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/CI_data">Data</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/CI_data">Data</a></li> | ||
| - | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/CI_discussion"> | + | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/CI_discussion">Modeling</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<li><a href="https://2011.igem.org/Team:NCTU_Formosa/CSP_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/CSP_design">Design</a></li> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/CSP_data">Data</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/CSP_data">Data</a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
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<li><a href="https://2011.igem.org/Team:NCTU_Formosa/BP_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/BP_design">Design</a></li> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/BP_data">Data</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/BP_data">Data</a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
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<li><a href="https://2011.igem.org/Team:NCTU_Formosa/VP_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/VP_design">Design</a></li> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/VP_data">Data</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/VP_data">Data</a></li> | ||
| - | |||
</ul> | </ul> | ||
</li> | </li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| - | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/modeling"> | + | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/modeling">Measurements</a></li> |
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/parts">Parts</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/parts">Parts</a></li> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/safty">Safety</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/safty">Safety</a></li> | ||
| - | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/contributions"> | + | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/humanpractice">Human Practice</a></li> |
| + | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/contributions">Attribution</a></li> | ||
<li><a class="arrow no-click">Notebook </a> | <li><a class="arrow no-click">Notebook </a> | ||
<ul> | <ul> | ||
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<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol">Mutation</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol">Mutation</a></li> | ||
| - | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol_F">Flow</a></li> | + | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol_F">Flow Cytometry</a></li> |
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol_G">GC</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol_G">GC</a></li> | ||
</ul> | </ul> | ||
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</ul> | </ul> | ||
| - | <br><br>< | + | <br><br> |
| - | <div | + | <div id="blueBox"><p>Protocols</p></div> |
| + | <div id="Box"><h2> Gas chromatography (GC) Protocol | ||
| + | </h2> | ||
| + | |||
| + | <b>Method:</b> | ||
| + | <p>Culture samples were centrifuged (12500×g, 25°C, 5 min), and the supernatants were analyzed for isobutanol. Isobutanol products were analyzed by GC equipped with flame ionization detector(FID) and a 50-m Ultra-2 column (Hewlett Packard, USA). The separation of alcohol compounds was carried out with a EQUity capirally column (SUPELCO, 60 m; 0.32 mm inside diameter; 1.0 μm film thickness). Filtrated samples were injected in split injection mode (1:10 split ratio).Nitrogen (constant flow 1 mL/min) was used as a carrier gas. The temperature of the injector was 150 °C. The oven program was as follows: 40°C ramp to 100 °C at 20 °C/min, 100 °C for 1 min, ramp to 130 °C at 15 °C/min, 130°C for 4 min.</p> | ||
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Revision as of 17:27, 3 October 2011
Protocols
Gas chromatography (GC) Protocol
Method:Culture samples were centrifuged (12500×g, 25°C, 5 min), and the supernatants were analyzed for isobutanol. Isobutanol products were analyzed by GC equipped with flame ionization detector(FID) and a 50-m Ultra-2 column (Hewlett Packard, USA). The separation of alcohol compounds was carried out with a EQUity capirally column (SUPELCO, 60 m; 0.32 mm inside diameter; 1.0 μm film thickness). Filtrated samples were injected in split injection mode (1:10 split ratio).Nitrogen (constant flow 1 mL/min) was used as a carrier gas. The temperature of the injector was 150 °C. The oven program was as follows: 40°C ramp to 100 °C at 20 °C/min, 100 °C for 1 min, ramp to 130 °C at 15 °C/min, 130°C for 4 min.
