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Team:Grinnell/Recipes
From 2011.igem.org
(Difference between revisions)
| Line 27: | Line 27: | ||
<li>2g Bacto peptone</li> | <li>2g Bacto peptone</li> | ||
<li>1g Yeast extract</li> | <li>1g Yeast extract</li> | ||
| - | <li>1mL 1M | + | <li>1mL 1M MgSO<sub>4</sub>⋅7H<sub>2</sub>O</li> |
| - | <li>1mL 0.5M | + | <li>1mL 0.5M CaCl<sub>2</sub></li></ul> |
<h2><a name="Agar"></a>Agar Plates</h2> | <h2><a name="Agar"></a>Agar Plates</h2> | ||
Revision as of 02:18, 28 June 2011
L Broth
For 1L:- 10g Tryptone
- 5g Yeast extract
- 5g NaCl
PYE Broth
For 1L:- 2g Bacto peptone
- 1g Yeast extract
- 1mL 1M MgSO4⋅7H2O
- 1mL 0.5M CaCl2
Agar Plates
Add 15g Bacto-agar to 1L of liquid media before autoclaving.Antibiotics Kitchen
| Antibiotic | Color Code | Stock (mg/mL) | LB Plate Conc (μg/mL) | PYE Plate Conc (μg/mL) |
|---|---|---|---|---|
| Ampicillin (amp) | Red | 50 | 100 | 50 |
| Carbenicillin (carb) | Red | 50 | 50 | 50 |
| Chloramphenicol (chlor) | 2 Orange | 5 (in EtOH) | 30 | 1 |
| Kanamycin (kan) | blue | 50 | 50 | 25 |
| Nalidixic acid (nal) | Green | 10 | 20 | 20 |
| Tetracycline (tet) | Black | 2 (in EtOH) | 12 | – |
| Oxytetracycline (oxytet) | Black | 2 (in EtOH) | − | 2 |
| Spectinomycin (spec) | Purple | 20 | 50 | 100 |
Note: for liquid cultures in PYE broth, use half the concentration that would be used for a PYE plate (LB remains the same for liquid and sold cultures).
