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Team:UC Davis/Notebook wip
From 2011.igem.org
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== Week 1== | == Week 1== | ||
| + | Monday | ||
| + | |||
| + | Today we had our first real day of lab work. We settled ourselves into the lab bench that we will work at for the summer and set up our area. Along with familiarizing the new members with lab protocols, we had a productive day of hydrating and transforming. We really liked the linearized vectors that the Registry sent along with the plates. | ||
| + | |||
-Rehydrated parts from 2011 Registry Distribution: | -Rehydrated parts from 2011 Registry Distribution: | ||
*-R0040=Tet promoter in psb1a2 | *-R0040=Tet promoter in psb1a2 | ||
| Line 104: | Line 108: | ||
*-B0015=Double terminator in psb1ak3 | *-B0015=Double terminator in psb1ak3 | ||
-Transformed all hydrated parts | -Transformed all hydrated parts | ||
| + | |||
| + | Tuesday | ||
| + | Today we tested out error-prone PCR | ||
-Cultured all parts and digested to check on a gel. All parts checked out. Sent out all hydrated parts to get sequenced. | -Cultured all parts and digested to check on a gel. All parts checked out. Sent out all hydrated parts to get sequenced. | ||
-Ligated mutants into screening plasmid to see if our error-prone PCRs had visible mutants. | -Ligated mutants into screening plasmid to see if our error-prone PCRs had visible mutants. | ||
| - | -Ordered primers for mutagenesis of LacI promoter, cI promoter and tet promoter. Also ordered primers to replace RBS (Bba_B0032) in Bba_E0240 to Bba_B0034. | + | |
| + | Wednesday | ||
| + | -Ordered primers for mutagenesis of LacI promoter, cI promoter and tet promoter. Also ordered primers to replace RBS (Bba_B0032) in Bba_E0240 to Bba_B0034. | ||
| + | |||
| + | Thursday | ||
| + | - | ||
== Week 2== | == Week 2== | ||
Revision as of 00:38, 28 June 2011
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Section 1
Mauris mauris ante, blandit et, ultrices a, suscipit eget, quam. Integer ut neque. Vivamus nisi metus, molestie vel, gravida in, condimentum sit amet, nunc. Nam a nibh. Donec suscipit eros. Nam mi. Proin viverra leo ut odio. Curabitur malesuada. Vestibulum a velit eu ante scelerisque vulputate.
Section 2
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Section 3
Nam enim risus, molestie et, porta ac, aliquam ac, risus. Quisque lobortis. Phasellus pellentesque purus in massa. Aenean in pede. Phasellus ac libero ac tellus pellentesque semper. Sed ac felis. Sed commodo, magna quis lacinia ornare, quam ante aliquam nisi, eu iaculis leo purus venenatis dui.
- List item one
- List item two
- List item three
Section 4
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Suspendisse eu nisl. Nullam ut libero. Integer dignissim consequat lectus. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos.
Contents |
Week 1
Monday
Today we had our first real day of lab work. We settled ourselves into the lab bench that we will work at for the summer and set up our area. Along with familiarizing the new members with lab protocols, we had a productive day of hydrating and transforming. We really liked the linearized vectors that the Registry sent along with the plates.
-Rehydrated parts from 2011 Registry Distribution:
- -R0040=Tet promoter in psb1a2
- -C0040=Tet repressor in psb1a2
- -C0012=LacI repressor in psb1a2
- -C0051=Lambda cI repressor in psb1a2
- -R0051=cI-regulated promoter in psb1a2
- -I732006=LacZ-alpha in psb1ak3
- -R0010=LacI regulated promoter in psb1a2
- -E0040=GFP coding region in psb1a2
- -J23101=Constitutive promoter in j61002
- -C0080=AraC repressor/activator in psb2k3
- -I13458=pC+AraC in psb1a3
- -I13453=pBAD promoter in psb1a3
- -B0015=Double terminator in psb1ak3
-Transformed all hydrated parts
Tuesday Today we tested out error-prone PCR -Cultured all parts and digested to check on a gel. All parts checked out. Sent out all hydrated parts to get sequenced. -Ligated mutants into screening plasmid to see if our error-prone PCRs had visible mutants.
Wednesday -Ordered primers for mutagenesis of LacI promoter, cI promoter and tet promoter. Also ordered primers to replace RBS (Bba_B0032) in Bba_E0240 to Bba_B0034.
Thursday -
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
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