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Team:KIT-Kyoto/ぷろとこる英語
From 2011.igem.org
(Difference between revisions)
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| - | Ligation<BR> | + | <p><strong>Ligation</strong> |
| + | <BR> | ||
<BR> | <BR> | ||
Refer to following table, prepare a reaction solution.<BR> | Refer to following table, prepare a reaction solution.<BR> | ||
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<tr><td width="130px" align=center>insert</td><td width="70px" align=right>0.5 µl</td></tr> | <tr><td width="130px" align=center>insert</td><td width="70px" align=right>0.5 µl</td></tr> | ||
<tr><td align=center>vector</td><td align=right>0.5 µl</td></tr> | <tr><td align=center>vector</td><td align=right>0.5 µl</td></tr> | ||
| - | <tr><td align=center> | + | <tr><td align=center>2 x Buffer</td><td align=right>2.5 µl</td></tr> |
<tr><td align=center>T4 ligase</td><td align=right>0.5 µl</td></tr> | <tr><td align=center>T4 ligase</td><td align=right>0.5 µl</td></tr> | ||
<tr><td align=center>H<sub>2</sub>O</td><td align=right>1.0 µl</td></tr> | <tr><td align=center>H<sub>2</sub>O</td><td align=right>1.0 µl</td></tr> | ||
Revision as of 13:17, 2 October 2011
いんぐりっしゅ！
Ligation
Refer to following table, prepare a reaction solution.
| insert | 0.5 µl |
| vector | 0.5 µl |
| 2 x Buffer | 2.5 µl |
| T4 ligase | 0.5 µl |
| H2O | 1.0 µl |
| total 5 µl |
Incubate it for 30min at 16 ℃.
