Team:IIT Madras/Notebook/Protocols/Preperation of antibiotics stock

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<h1>Protocols</h1><br/>
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<h1>Preperation of antibiotics stock</h1><br/>
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<ul>
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<table><tr><td>Antibiotic</td><td>Conc. (1000x)</td><td>Solvent</td></tr>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Preperation_of_competent_cells">Preperation of competent DH5alpha cells</a></li>  
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<tr><td>Ampicillin</td><td>100 mg/ml</td><td>Water</td></tr>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Miniprep_using_kits">Miniprep using kit</a></li>  
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<tr><td>Chloramphenicol</td><td>34 mg/ml</td><td>Ethanol</td></tr>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Gel_elusion">Gel Elusion</a></li>
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<tr><td>Kanamycin</td><td>50 mg/ml</td><td>Water</td></tr>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Transformation">Transformation</a></li>
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</table>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Miniprep_using_alkaline_lysis_buffers">Miniprep using alkaline lysis buffers</a></li>
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<p><b>Filter sterilize them</b><br/><br/>
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</ul>
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Add 1 ul for each ml of media (Applicable for all the above antibiotics).</p>
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Revision as of 10:38, 2 October 2011

bar iGEM 2011 - Home Page Indian Institute of Technology - Madras

Preperation of antibiotics stock


AntibioticConc. (1000x)Solvent
Ampicillin100 mg/mlWater
Chloramphenicol34 mg/mlEthanol
Kanamycin50 mg/mlWater

Filter sterilize them

Add 1 ul for each ml of media (Applicable for all the above antibiotics).