Team:Peking S/lab/notebook/wdq

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* <span style="font-size:4mm;">[[Team:Peking_S/lab/notebook/wdq#October| October, 2011]]</span>
* <span style="font-size:4mm;">[[Team:Peking_S/lab/notebook/wdq#October| October, 2011]]</span>
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==June==
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|style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#6.22 - 6.25|22]]
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[<html><a href="#top">TOP</a></html>]
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===6.22 - 6.25===
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Design primers.
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===6.26 - 6.27===
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Culture streptomycin griseus strain, which is the source of afsA, arpA, padpA.
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===6.28===
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afsA, arpA PCR using different annealing temperature, with gradient 4 ℃.
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Identify them by 1% agarose gel electrophoresis.
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Excise the gel slice and extract the fragments.
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Second PCR using the products of gel extraction.
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Electrophoresis PCR reaction system in 1.5% agarose gel.
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Double digest the products of gel extraction by EcoR1-HF and Xba1.
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Double digest the plasmid of B0015 by EcoR1-HF and Spe1.
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Ligase afsA and arpA to the vector  of B0015.
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===6.29===
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Transform the ligation reaction system.
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Sythesis the genes of CAI-1 system using DNAWorks assembly and PadpA PCR.
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Identify them by 1% agarose gel electrophoresis.
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Excise the gel slice of pqrr, J23106+B0034+luxU, PadpA and extract the fragments.
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ligase the products of gel extraction to the vector pEASY-Blunt.
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===6.30===
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Transform the ligation reaction system.
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Pick six clones each plate and shave at 37℃ to amplify the bacteria.

Revision as of 16:51, 1 October 2011

Contents

summary

blahblah...

Contents


June

Mon Tue Wed Thu Fri Sat Sun
- - 1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 - - -

[TOP]

6.22 - 6.25

Design primers.

6.26 - 6.27

Culture streptomycin griseus strain, which is the source of afsA, arpA, padpA.

6.28

afsA, arpA PCR using different annealing temperature, with gradient 4 ℃.

Identify them by 1% agarose gel electrophoresis.

Excise the gel slice and extract the fragments.

Second PCR using the products of gel extraction.

Electrophoresis PCR reaction system in 1.5% agarose gel.

Double digest the products of gel extraction by EcoR1-HF and Xba1.

Double digest the plasmid of B0015 by EcoR1-HF and Spe1.

Ligase afsA and arpA to the vector of B0015.

6.29

Transform the ligation reaction system.

Sythesis the genes of CAI-1 system using DNAWorks assembly and PadpA PCR.

Identify them by 1% agarose gel electrophoresis.

Excise the gel slice of pqrr, J23106+B0034+luxU, PadpA and extract the fragments.

ligase the products of gel extraction to the vector pEASY-Blunt.

6.30

Transform the ligation reaction system.

Pick six clones each plate and shave at 37℃ to amplify the bacteria.

Retrieved from "http://2011.igem.org/Team:Peking_S/lab/notebook/wdq"