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Team:WHU-China/Notebook
From 2011.igem.org
(Difference between revisions)
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<a href="https://2011.igem.org/Team:WHU-China/Notebook/Protocols"><img style="position:absolute;top:380px;right:100px;width:90px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a> | <a href="https://2011.igem.org/Team:WHU-China/Notebook/Protocols"><img style="position:absolute;top:380px;right:100px;width:90px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a> | ||
</div> | </div> | ||
| + | |||
| + | |||
<div id="daytoday"> | <div id="daytoday"> | ||
<img src="/wiki/images/8/83/Whu-Progress.png"/> | <img src="/wiki/images/8/83/Whu-Progress.png"/> | ||
| - | + | <img src="/wiki/images/5/50/Whu-system.jpg" style="position:absolute;left:20px;top:40px;width:300px;height:360px;"> | |
| - | + | <div style="position:absolute;top:60px;right:30px;width:620px;font-size:15px;"> | |
| - | + | • Dealing with biobricks</br> | |
| + | • Preparation of competent cells (Using Calcium Chloride)</br> | ||
| + | • Plasmid Extraction（Using Plasmid Mini Kit）</br> | ||
| + | • Transformation (heat-shock method)</br> | ||
| + | • Cleavage with two restriction enzymes</br> | ||
| + | • Get Extraction</br> | ||
| + | • Identification of DNA by Electrophoresis</br> | ||
| + | • Ligation</br> | ||
| + | • PCR of the colony</br> | ||
| + | • Identification of DNA by Sequencing</br> | ||
| + | • Others............ | ||
| + | </div> | ||
<a href="https://2011.igem.org/Team:WHU-China/Notebook/Daytoday"><img style="position:absolute;top:380px;right:100px;width:90px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a> | <a href="https://2011.igem.org/Team:WHU-China/Notebook/Daytoday"><img style="position:absolute;top:380px;right:100px;width:90px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a> | ||
</div> | </div> | ||
Revision as of 08:37, 30 September 2011
• Dealing with biobricks
• Preparation of competent cells (Using Calcium Chloride)
• Plasmid Extraction（Using Plasmid Mini Kit）
• Transformation (heat-shock method)
• Cleavage with two restriction enzymes
• Get Extraction
• Identification of DNA by Electrophoresis
• Ligation
• PCR of the colony
• Identification of DNA by Sequencing
• Others............
• Dealing with biobricks
• Preparation of competent cells (Using Calcium Chloride)
• Plasmid Extraction（Using Plasmid Mini Kit）
• Transformation (heat-shock method)
• Cleavage with two restriction enzymes
• Get Extraction
• Identification of DNA by Electrophoresis
• Ligation
• PCR of the colony
• Identification of DNA by Sequencing
• Others............
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