"
Team:Kyoto
From 2011.igem.org
| Line 15: | Line 15: | ||
<div> | <div> | ||
== <html><a href="https://2011.igem.org/Team:Kyoto/Hunger">Project Hunger</a></html> == | == <html><a href="https://2011.igem.org/Team:Kyoto/Hunger">Project Hunger</a></html> == | ||
| - | |||
It is a burden for the E.coli to emit light. | It is a burden for the E.coli to emit light. | ||
This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC, which activate a certain promoter under the condition that supply of nitrogen is not enough. | This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC, which activate a certain promoter under the condition that supply of nitrogen is not enough. | ||
| Line 22: | Line 21: | ||
<div> | <div> | ||
== <html><a href="https://2011.igem.org/Team:Kyoto/Luminescence">Project Luminescence</a></html> == | == <html><a href="https://2011.igem.org/Team:Kyoto/Luminescence">Project Luminescence</a></html> == | ||
| - | |||
There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone. | There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone. | ||
Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from | Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from | ||
| Line 30: | Line 28: | ||
<div> | <div> | ||
== <html><a href="https://2011.igem.org/Team:Kyoto/Digestion">Project Digestion</a></html> == | == <html><a href="https://2011.igem.org/Team:Kyoto/Digestion">Project Digestion</a></html> == | ||
| - | |||
In this part, we aimed to create ''E.coli'' which digest bugs and survive under the nitrogen-poor condition. So, we decided to use SAM-P20 and ChiA genes, which encode protease and chitinase. In order to measure the activity of each enzyme correctly, we examined assay methods first. | In this part, we aimed to create ''E.coli'' which digest bugs and survive under the nitrogen-poor condition. So, we decided to use SAM-P20 and ChiA genes, which encode protease and chitinase. In order to measure the activity of each enzyme correctly, we examined assay methods first. | ||
</div> | </div> | ||
</div> | </div> | ||
| + | <html> | ||
| + | <style type="text/css"> | ||
| + | #project_panel { | ||
| + | width: 773px; | ||
| + | margin: 0px; | ||
| + | padding: 0px; | ||
| + | } | ||
| + | |||
| + | #project_panel div { | ||
| + | width: 250px; | ||
| + | margin: 0px; | ||
| + | padding: 3px; | ||
| + | float: left; | ||
| + | } | ||
| + | </style> | ||
| + | </html> | ||
<!-- end project_panel --> | <!-- end project_panel --> | ||
| Line 46: | Line 59: | ||
==Reference== | ==Reference== | ||
</div> | </div> | ||
| + | <html> | ||
| + | <style type="text/css"> | ||
| + | #under_box { | ||
| + | width: 773px; | ||
| + | margin: 0px; | ||
| + | padding: 0px; | ||
| + | } | ||
| + | #under_box div { | ||
| + | width: 360px; | ||
| + | margin: 0px; | ||
| + | padding: 9px; | ||
| + | float: left; | ||
| + | } | ||
| + | </style> | ||
| + | </html> | ||
</div> | </div> | ||
| + | <!-- end under_box -> | ||
Revision as of 03:09, 30 September 2011
Contents |
Summary
We create new E.coli which hunt and eat insects. it is named Carnivorous E.coli. Carnivorous E.coli emits light when hungry, and insects come near it attracted by the light. Then, it secretes viscous material to catch the insects. The insects caught are solved by protease and chitinase that Carnivorous E.coli secretes also. So, Carnivorous E.coli can hunt!
Project Hunger
It is a burden for the E.coli to emit light. This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC, which activate a certain promoter under the condition that supply of nitrogen is not enough.
Project Luminescence
There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone. Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from 2010 Cambridge.
Project Digestion
In this part, we aimed to create E.coli which digest bugs and survive under the nitrogen-poor condition. So, we decided to use SAM-P20 and ChiA genes, which encode protease and chitinase. In order to measure the activity of each enzyme correctly, we examined assay methods first.
