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Team:WHU-China/Notebook
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<a href="https://2011.igem.org/wiki/index.php?title=Team:WHU-China/Team/History&action=edit"><img class="whucontact" style="position:relative;left:636px;top:38px;z-index:10;" src="/wiki/images/2/2a/Whu-Home_r1_c1.png"/></a> | <a href="https://2011.igem.org/wiki/index.php?title=Team:WHU-China/Team/History&action=edit"><img class="whucontact" style="position:relative;left:636px;top:38px;z-index:10;" src="/wiki/images/2/2a/Whu-Home_r1_c1.png"/></a> | ||
Revision as of 11:01, 29 September 2011
• Dealing with biobricks
• Preparation of competent cells (Using Calcium Chloride)
• Plasmid Extraction（Using Plasmid Mini Kit）
• Transformation (heat-shock method)
• Cleavage with two restriction enzymes
• Get Extraction
• Identification of DNA by Electrophoresis
• Ligation
• PCR of the colony
• Identification of DNA by Sequencing
• Others............
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