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Team:WHU-China/Notebook
From 2011.igem.org
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<img src="/wiki/images/6/6f/Whu-Protocols.png"/> | <img src="/wiki/images/6/6f/Whu-Protocols.png"/> | ||
<img src="https://static.igem.org/mediawiki/2011/c/c6/Whu-protologo1.png" style="position:absolute;left:20px;top:40px;width:300px;height:360px;"> | <img src="https://static.igem.org/mediawiki/2011/c/c6/Whu-protologo1.png" style="position:absolute;left:20px;top:40px;width:300px;height:360px;"> | ||
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• Dealing with biobricks</br> | • Dealing with biobricks</br> | ||
• Preparation of competent cells (Using Calcium Chloride)</br> | • Preparation of competent cells (Using Calcium Chloride)</br> | ||
Revision as of 10:02, 29 September 2011
• Dealing with biobricks
• Preparation of competent cells (Using Calcium Chloride)
• Plasmid Extraction（Using Plasmid Mini Kit）
• Transformation (heat-shock method)
• Cleavage with two restriction enzymes
• Get Extraction
• Identification of DNA by Electrophoresis
• Ligation
• PCR of the colony
• Identification of DNA by Sequencing
• Others............
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