Team:UTP-Panama/Results

Spectrophotometry Results

• It grows well @23°C (best in Minimal Media than LB)
• It shows almost no growth @8°C

Discussion of Results

As we expected, our Renbo grew well at 37°C, grew regular under cold shock stress in LB (23°C) and did not grow at 8°C.
The BBa_381001 grew well alone at 23°C, better than the BBa_410000 and our device (expected to work better).

Looking at the results for BBa_410000, it decreases the capability of BBa_381001, under a cold shock.

The next step in our project:
Continuing with our original design, we are are planning to use another promoter as the CspA in stead of HybB, that improve the gene expression under cold shock. We will keep experimenting with our device.


It´s ok for us first year with students without Life sciences background!
VIVA iGEM! We learn a lot.... see you in Indiana and MIT

Graphs:

Discussion of Results

As we expected, our Renbo grew well at 37°C, grew regular under cold shock stress in LB (23°C) and did not grow at 8°C.
The BBa_381001 grew well alone at 23°C, better than the BBa_410000 and our device (expected to work better).

Looking at the results for BBa_410000, it decreases the capability of BBa_381001, under a cold shock.

The next step in our project:
Continuing with our original design, we are are planning to use another promoter as the CspA in stead of HybB, that improve the gene expression under cold shock. We will keep experimenting with our device.


It´s ok for us first year with students without Life Sciences background!