Team:Calgary/Notebook/Protocols/Process20

From 2011.igem.org

(Difference between revisions)
 
Line 8: Line 8:
   <ul>
   <ul>
     <li> Overnight bacterial growth </li>
     <li> Overnight bacterial growth </li>
-
     <li> screw cap tubes </li>
+
     <li> Screw Cap Tubes </li>
-
     <li> glycerol </li>
+
     <li> Glycerol </li>
   </ul>
   </ul>
   <p> Protocol </p>
   <p> Protocol </p>

Latest revision as of 04:13, 29 September 2011


Preparing Glycerol Stocks

Reagents and Materials

  • Overnight bacterial growth
  • Screw Cap Tubes
  • Glycerol

Protocol

  1. Pipet 0.5mL of 50% glycerol into 3 1.5mL screw cap tubes
  2. Add 0.5mL of overnight culture to each tube.
  3. Pipet up and down to gently mix.
  4. Flash freeze in liquid N2 or dry ice/ethanol bath.
  5. Place in -80°C freezer when frozen.








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