Team:Calgary/Notebook/Protocols/Process5
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- | This protocol is used to prepare | + | This protocol is used to prepare f2 media for algal growth.</p> |
<h4> Stock solutions </h4> | <h4> Stock solutions </h4> | ||
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<li>NaNO3 (150.0 g/L)</li> | <li>NaNO3 (150.0 g/L)</li> | ||
<li>Trace metals (mixed to 750 mL to dissolve, and then brought up to 1 L): CuSO4.5H2O (19.6 mg/L), ZnSO4.7H2O (44.0 mg/L), CoCl2.6H2O (22.0 mg/L), MnCl2.4H2O (360.0 mg/L), Na2MoO4.2H2O (12.6 mg/L) </li> | <li>Trace metals (mixed to 750 mL to dissolve, and then brought up to 1 L): CuSO4.5H2O (19.6 mg/L), ZnSO4.7H2O (44.0 mg/L), CoCl2.6H2O (22.0 mg/L), MnCl2.4H2O (360.0 mg/L), Na2MoO4.2H2O (12.6 mg/L) </li> | ||
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<h4> Procedure </h4> | <h4> Procedure </h4> |
Revision as of 03:26, 29 September 2011
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Preparation of f2 Media for Algae
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This protocol is used to prepare f2 media for algal growth.
Stock solutions
Procedure
- Add 0.5 mL of each of stock solutions 1-5 (listed below) to 1 L of seawater
- Split into flasks and autoclave at 121 °C (15 PSI) for 15 minutes
- Prepare phosphate solution (see below) and autoclave dilute phosphate stock at 121°C (15PSI, 15 mins). After cooling, dispense aseptically with sterilised automatic dispenser. This component is autoclaved separately in order to prevent precipitation
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