"
Team:ITESM Mexico/Extras
From 2011.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
[[File:Giant_microbe.png|center]] | [[File:Giant_microbe.png|center]] | ||
| - | ===Home made quick saliva DNA extraction protocol:=== | + | ==='''Home made quick saliva DNA extraction protocol:'''=== |
::#Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass | ::#Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass | ||
Revision as of 03:19, 29 September 2011
ITESM MÉXICO
SensE.coli
Extras
- Some aditional stuff.
Home made quick saliva DNA extraction protocol:
- Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass
- Prepare a solution with soap and water in a beaker
- Add the soap solution to the last rinse
- Add frozen (0°C) etanol to the last rinse
- Observe how a DNA thread forms, take it and place it in an eppendorf tube.
Abbreviations and glossary:
- Biobrickoatl:It's the biobrick deity for the Mesoamerican cultures, although at that time they didn't knew what a biobrick meant. The great feathered biobrick serpent vigilates over all new and old biobricks.
- Competent cells: Cells that can incorporate, in an easier way, foreign DNA into their cell walls.
- CcaS-EnvZ: Is the green light receptor that induces the expresión of phycobilisome linker protein.
- CFP: Cyan Fluorescent Protein
- Crst: Lock for the PbadST (strong, for low concentrations) promoter
- CrWk: Lock for the Pbad WK (weak, for high concentration) promoter
- GFP: Green Fluorescent Protein
- ho1: Heme oxigenase 1
- iTaST: Inverse TaST, complementary sequence to the Key TaST, blocks TaST activity
- pBADST: Strong arabinose promoter for low concentration detection.
- pBADWk: Weak arabinose promoter for high concentration detection.
- Pcy4A: Phycocyanobilin ferredoxin oxidoreductase.
- RBS: Ribosome binding site
- TaST: Key for the CrST lock
- TaWk: Key for the CrWk lock
- Term: Terminator
