From 2011.igem.org
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| - | We take a sample of rh1AB purifying gen without mutation and doing a PCR because doesn’t have a lot and we have to digest with Pst1. On Monday we are going to do a mutagenesis.
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| - | We use the PCR protocol that functions well with our gene ([https://2011.igem.org/PCR'''PCR Protocol''']) and we test all primers because we don’t know which primers the team used the last year for genes amplification.
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| - | [[File:tabla7.png|700px|thumb|left|alt text]]
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| - | Primers:
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| - | 1. F:RhTf2a R:RhTR2a
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| - | 2. F:RhT-f1b R:RhT-2b
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| - | 3. F:RhTBio-R2a R:RhTBio-F1a
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| - | 4. F:RhTBio-f1 R:RhTBio-2b
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| - | Meanwhile Orlando and Paul make the miniprep of (RBS + P) and (GFP + T) using the protocol of dirty miniprep on page 80.Right Now Sergio and Natasha take the miniprep results and check the concentration with Nanodrop while Paul prepares PCR.
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| - | RESULTS:
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| - | [[File:tabla8.png|700px|thumb|left|alt text]]
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| - | We have good levels of absorbance and concentration in the samples, we hope that the electrophoresis be good.
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Revision as of 02:35, 29 September 2011
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