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Team:Panama/13 July 2011
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(Created page with "=='''July 13, 2011'''== '''We did:''' 1. Electrophoresis of the products of yesterday PCR and 3 digestions. '''What we expect to know?''' If EcoR1 enzyme digest and in how muc...")
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(Created page with "=='''July 13, 2011'''== '''We did:''' 1. Electrophoresis of the products of yesterday PCR and 3 digestions. '''What we expect to know?''' If EcoR1 enzyme digest and in how muc...")
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Revision as of 02:29, 29 September 2011
July 13, 2011
We did:
1. Electrophoresis of the products of yesterday PCR and 3 digestions.
What we expect to know? If EcoR1 enzyme digest and in how much microliters is efficient, that with dilution the amplification be more effective.
Results: We had not results of PCR :( !
Apparently EcoR1 enzyme cut the plasmid, we see bands of approximately 2 kb and others of 1,2 kb. We think that the gen isn't in the plasmid because the weight desired es of 4,4 kb (4,441pb)
