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Team:UC Davis/Project
From 2011.igem.org
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We set out to develop a rugged process for the rapid production of mutant libraries of any <a href="http://biobricks.org/" target:"_blank">BioBrick</a> part using standard primers and a <a href="https://2011.igem.org/Team:UC_Davis/Protocols#ER-PCR">simple mutagenic PCR protocol</a>. We chose to prototype this process by creating mutant libraries of the LacI, TetR and λ cI repressible promoters and to mutate GFP to visually assess our ability to create functional protein mutants.<br><br> | We set out to develop a rugged process for the rapid production of mutant libraries of any <a href="http://biobricks.org/" target:"_blank">BioBrick</a> part using standard primers and a <a href="https://2011.igem.org/Team:UC_Davis/Protocols#ER-PCR">simple mutagenic PCR protocol</a>. We chose to prototype this process by creating mutant libraries of the LacI, TetR and λ cI repressible promoters and to mutate GFP to visually assess our ability to create functional protein mutants.<br><br> | ||
| - | As of October 2011, we have a functioning mutant library generation and screening process, and a selection of well-characterized promoter mutants, including seven LacI | + | As of October 2011, we have a functioning mutant library generation and screening process, and a selection of well-characterized promoter mutants, including seven LacI promoter mutants. We also have nine TetR promoter mutants, seven λ cI promoter mutants, and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization. |
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Revision as of 02:17, 29 September 2011
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Overview
We set out to develop a rugged process for the rapid production of mutant libraries of any BioBrick part using standard primers and a simple mutagenic PCR protocol. We chose to prototype this process by creating mutant libraries of the LacI, TetR and λ cI repressible promoters and to mutate GFP to visually assess our ability to create functional protein mutants.
As of October 2011, we have a functioning mutant library generation and screening process, and a selection of well-characterized promoter mutants, including seven LacI promoter mutants. We also have nine TetR promoter mutants, seven λ cI promoter mutants, and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.
As of October 2011, we have a functioning mutant library generation and screening process, and a selection of well-characterized promoter mutants, including seven LacI promoter mutants. We also have nine TetR promoter mutants, seven λ cI promoter mutants, and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.
