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Team:ITESM Mexico/Parts submitted
From 2011.igem.org
Maqengel 90 (Talk | contribs) |
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| - | ''' | + | ::*'''RecA''' |
| + | ::Protein coding sequence | ||
Sequence | Sequence | ||
| - | |||
'''GAATTCGCGGCCGCTTCTAG'''ATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATT | '''GAATTCGCGGCCGCTTCTAG'''ATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATT | ||
GAGAAACAATTTGGTAAAGGCTCCATCATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATAT | GAGAAACAATTTGGTAAAGGCTCCATCATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATAT | ||
| Line 82: | Line 82: | ||
| + | ::*'''Crxst''' | ||
| - | + | ::This DNA sequence acts as a lock for the low arabinose concentration regulation path. | |
| - | + | ||
| - | This DNA sequence acts as a lock for the low arabinose concentration regulation path. | + | |
Sequence | Sequence | ||
| - | |||
'''GAATTCGCGGCCGCTTCTAGAG'''AAGCGCAGTAATTCACCTCTTGGATTTGGGTATCA'''TACTAGTAGCGGCCGCTGCAG''' | '''GAATTCGCGGCCGCTTCTAGAG'''AAGCGCAGTAATTCACCTCTTGGATTTGGGTATCA'''TACTAGTAGCGGCCGCTGCAG''' | ||
| - | ''' | + | ::*'''Tast''' |
| - | This sequence acts as the key for the Crxst lock and it unlocks it, in order to activate following mechanism. | + | ::This sequence acts as the key for the Crxst lock and it unlocks it, in order to activate following mechanism. |
Sequence | Sequence | ||
| - | |||
'''GAATTCGCGGCCGCTTCTAGAG'''AGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGAACCTAAAGGGATAGTT | '''GAATTCGCGGCCGCTTCTAGAG'''AGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGAACCTAAAGGGATAGTT | ||
'''TACTAGTAGCGGCCGCTGCAG''' | '''TACTAGTAGCGGCCGCTGCAG''' | ||
| Line 104: | Line 101: | ||
| - | ''' | + | ::*'''Tawk''' |
| - | This sequence acts as the key fot Crxwk lock and it unlocks it, in order to activate the following mechanism. | + | ::This sequence acts as the key fot Crxwk lock and it unlocks it, in order to activate the following mechanism. |
Sequence | Sequence | ||
| - | |||
'''GAATTCGCGGCCGCTTCTAGAG'''AGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGATAAACCGTAAGGGTT | '''GAATTCGCGGCCGCTTCTAGAG'''AGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGATAAACCGTAAGGGTT | ||
'''TACTAGTAGCGGCCGCTGCAG''' | '''TACTAGTAGCGGCCGCTGCAG''' | ||
| + | ::*'''Crxwk''' | ||
| - | + | ::This DNA sequence acts as a lock for the high arabinose concentration regulation path. | |
| - | + | ||
| - | This DNA sequence acts as a lock for the high arabinose concentration regulation path. | + | |
Sequence | Sequence | ||
| - | |||
'''GAATTCGCGGCCGCTTCTAGAG'''AAGCGCAGTAATTCACCTCTATTTGGCATTCCCAA'''TACTAGTAGCGGCCGCTGCAG''' | '''GAATTCGCGGCCGCTTCTAGAG'''AAGCGCAGTAATTCACCTCTATTTGGCATTCCCAA'''TACTAGTAGCGGCCGCTGCAG''' | ||
| - | ''' | + | ::*'''Itast''' |
| - | This is a complementary sequence for the Tast key, so we call it the antikey since it will inactivate the Tast key and preventing it to interact with the Crxst lock. | + | ::This is a complementary sequence for the Tast key, so we call it the antikey since it will inactivate the Tast key and preventing it to interact with the Crxst lock. |
Sequence | Sequence | ||
Revision as of 01:59, 29 September 2011
ITESM MÉXICO
SensE.coli
Part Registry
| Name | Description | Type | Designer | Length | Lenght (prefix;suffix) |
| BBa_K587000 | RecA | Protein coding | David Maycotte/Patricia Campos | 1062 | 1103 |
| BBa_K587001 | Crxst | Regulatory | David Maycotte | 35 | 78 |
| BBa_K587002 | Tast | Regulatory | David Maycotte | 80 | 123 |
| BBa_K587003 | Tawk | Regulatory | David Maycotte | 79 | 122 |
| BBa_K587004 | Crxwk | Regulatory | David Maycotte | 35 | 78 |
| BBa_K587005 | Itast | Regulatory | David Maycotte | 81 | 124 |
== Sequences and description =='
- RecA
- Protein coding sequence
Sequence GAATTCGCGGCCGCTTCTAGATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATT GAGAAACAATTTGGTAAAGGCTCCATCATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATAT CGCGCTTGGGGCAGGTGGTCTGCCGATGGGCCGTATCGTCGAAATCTACGGACCAGAATCTTCCGGTAAAACCACGCTGACGTTGCAGGTGATCGCCGC AGCGCAGCGCGAAGGTAAAACCTGTGCGTTTATCGATGCTGAACACGCGCTGGACCCAATCTACGCACGTAAACTGGGCGTCGATATTGACAACCTGCT GTGCTCCCAGCCGGATACCGGCGAGCAGGCACTGGAAATCTGTGATGCCCTGGCACGTTCTGGCGCAGTAGACGTTATCGTCGTTGACTCCGTGGCGGCAC TGACGCCGAAAGCGGAAATCGAAGGCGAAATCGGCGACTCTCACATGGGCCTTGCGGCACGTATGATGAGCCAGGCGATGCGTAAGCTGGCGGGTAACCTG AAGCAGTCCAACACGCTGCTGATCTTCATCAACCAGATCCGTATGAAAATTGGTGTGATGTTCGGTAACCCGGAAACCACTACCGGTGGTAACGCGCTGAAA TTCTACGCCTCTGTTCGTCTCGACATCCGTCGTATCGGCGCGGTGAAAGAGGGCGAAAACGTGGTGGGTAGCGAAACCCGCGTGAAAGTGGTGAAGAACAAA ATCGCTGCGCCGTTTAAACAGGCTGAGTTCCAGATCCTCTACGGCGAAGGTATCAACTTCTACGGCGAGCTGGTTGACCTGGGCGTGAAAGAGAAGCTTATC GAGAAAGCAGGCGCGTGGTACAGCTACAAAGGTGAGAAGATCGGTCAGGGTAAAGCGAATGCGACTGCCTGGCTGAAAGATAACCCGGAAACCGCGAAAGAG ATCGAGAAGAAAGTACGTGAGTTGCTGCTGAGCAACCCGAACTCAACGCCGGATTTCTCTGTAGATGACAGCGAAGGCGTAGCAGAAACTAACGAAGATTT TTAATACTAGTAGCGGCCGCTGCAG
- Crxst
- This DNA sequence acts as a lock for the low arabinose concentration regulation path.
Sequence GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTTGGATTTGGGTATCATACTAGTAGCGGCCGCTGCAG
- Tast
- This sequence acts as the key for the Crxst lock and it unlocks it, in order to activate following mechanism.
Sequence GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGAACCTAAAGGGATAGTT TACTAGTAGCGGCCGCTGCAG
- Tawk
- This sequence acts as the key fot Crxwk lock and it unlocks it, in order to activate the following mechanism.
Sequence GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGATAAACCGTAAGGGTT TACTAGTAGCGGCCGCTGCAG
- Crxwk
- This DNA sequence acts as a lock for the high arabinose concentration regulation path.
Sequence GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTATTTGGCATTCCCAATACTAGTAGCGGCCGCTGCAG
- Itast
- This is a complementary sequence for the Tast key, so we call it the antikey since it will inactivate the Tast key and preventing it to interact with the Crxst lock.
Sequence
GAATTCGCGGCCGCTTCTAGAGTCTAGGAGAGATATATGGTATGGTGGTTAATTTTCATTAACCACCAAAGCGCAGTAATTCACCTCTTGGTTTCCCTATCAA TACTAGTAGCGGCCGCTGCAG
