Team:UT Dallas/data

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Revision as of 01:37, 29 September 2011

Data

Data For Our Favorite New Parts
Main Page PcstA-RBS-LuxI-double terminator, BBa_K569001: This part produces LuxI,an enzyme for creating acyl-homoserine lactones from normal cell metabolites, in the absence of glucose. This part is repressed by glucose.

Main Page PyeaR-ToxR-FGFR, BBa_K569013: PyeaR is a nitrate inducible promoter. ToxR+FGFR is a receptor fusion of two dimerizing proteins. FGFR responds to FGF by dimerizing; this causes ToxR to dimerize. When ToxR is dimerized it acts as a transcription factor for the ctx promoter.

Main Page SCP-ToxR-FGFR, BBa_K569014: This part constitutively produces the ToxR+FGFR fusion protein. ToxR+FGFR dimerizes in response to FGF and can then activate the ctx promoter from the bacteria Vibrio cholera. ToxR by itself is a transcription factor, while FGFR is a immune system sensor.

Data For Pre-existing Parts
Main Page SCP-ToxR-FGFR, BBa_K569014: This part constitutively produces the ToxR+FGFR fusion protein. ToxR+FGFR dimerizes in response to FGF and can then activate the ctx promoter from the bacteria Vibrio cholera. ToxR by itself is a transcription factor, while FGFR is a immune system sensor.

Experience We grew Pcst-RBS-LuxI-terminator and AHL-inducible ColicinE2-GFP transformed in BL21 E.coli cells. We combined the two cultures in various ratios and either added or did not add glucose. We allowed these samples to continue growing for 2 hours and we took measurements using a fluorescent microscope.

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 <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569001">Main Page</a> PcstA-RBS-LuxI-double terminator,  BBa_K569001: This part produces LuxI,an enzyme for creating acyl-homoserine lactones from normal cell metabolites, in the absence of glucose. This part is repressed by glucose.<br><br>
 <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569013">Main Page</a> PyeaR-ToxR-FGFR, BBa_K569013: PyeaR is a nitrate inducible promoter. ToxR+FGFR is a receptor fusion of two dimerizing proteins. FGFR responds to FGF by dimerizing; this causes ToxR to dimerize. When ToxR is dimerized it acts as a transcription factor for the ctx promoter. <br><br>
-<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569014">Main Page</a> SCP-ToxR-FGFR, BBa_K569014: This part constitutively produces the ToxR+FGFR fusion protein. ToxR+FGFR dimerizes in response to FGF and can then activate the ctx promoter from the bacteria Vibrio cholera. ToxR by itself is a transcription factor, while FGFR is a immune system sensor.<br><br><br>
+<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569014">Main Page</a> SCP-ToxR-FGFR, BBa_K569014: This part constitutively produces the ToxR+FGFR fusion protein. ToxR+FGFR dimerizes in response to FGF and can then activate the ctx promoter from the bacteria Vibrio cholera. ToxR by itself is a transcription factor, while FGFR is a immune system sensor.<br><br>
 <b>Data For Pre-existing Parts</b><br>
-Experience - Wintergreen odor enzyme generator, BBa_J45119 (MIT, iGEM 2006): 98 out of 100 volunteer subjects standing up to 5 feet away from the bacterial cultures could distinguish wintergreen-producing bacteria from negative controls.
+<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569014">Main Page</a> SCP-ToxR-FGFR, BBa_K569014: This part constitutively produces the ToxR+FGFR fusion protein. ToxR+FGFR dimerizes in response to FGF and can then activate the ctx promoter from the bacteria Vibrio cholera. ToxR by itself is a transcription factor, while FGFR is a immune system sensor.<br><br>
-Experience - RBS, BBa_J61110 (Arkin Lab, 2007): Of the 5 RBS Parts we tested, this RBS works best for expressing yellow fluorescent protein-tagged BAR
+<a href="http://partsregistry.org/Part:BBa_K131010:Experience">Experience</a> We grew Pcst-RBS-LuxI-terminator and AHL-inducible ColicinE2-GFP transformed in BL21 E.coli cells. We combined the two cultures in various ratios and either added or did not add glucose. We allowed these samples to continue growing for 2 hours and we took measurements using a fluorescent microscope.
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