Team:Queens Canada/Project/Methods

As C. elegans is a multi-celleular organism, developing a concrete approach to our project required extensive research and planning. This section outlines our general methodology to tackling the bioremediation concept.

After narrowing down our project idea, the first step we took was to research compounds and G-protein coupled receptors (GPCRs) our worm could use to detect pollutants. Since our team consisted of students from across Canada, members of our team were aware of the issues surrounding the development of the oil sands in Western Canada, which was part of the rationale for the project.

After narrowing down our project idea, the first step we took was to research compounds and G-protein coupled receptors (GPCRs) our worm could use to detect pollutants.

After narrowing down our project idea, the first step we took was to research compounds and G-protein coupled receptors (GPCRs) our worm could use to detect pollutants.

1. PharmacoInformatics Laboratory, GLIDA: GPCR Ligand Database (ver. 2.04, 2010), Kyoto University
2. Kiyohara H, Torigoe S, Kaida N, Asaki T, Iida, T, Hayashi H and Takizawa N. (1994) Cloning and Characterization of a Chromosomal Gene Cluster, pah, That Encodes the Upper Pathway for Phenanthrene and Naphthalene Utilization by Pseudomonas putida OUS82. Journal of Bacteriology, 176:2439-2443.
3. Ahmed S and Hodgkin J. (2000) MRT-2 checkpoint protein is required for germline immortality and telomere replication in C. elegans. Nature, 403(6766):149-151.