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Team:Tec-Monterrey/projectnotebook may/day20
From 2011.igem.org
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20/May/2011 | 20/May/2011 | ||
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| - | • DNA | + | • DNA digestion with SspI and NdeI + Buffer 3 at 37 C , 1 hr. |
</br> | </br> | ||
• Electrophoresis 60 V, 1.5 dr. | • Electrophoresis 60 V, 1.5 dr. | ||
</br> | </br> | ||
| - | • Pre- inocolum of virgin cells in 15-20 microliters of LB without antibiotic | + | • Pre-inocolum of virgin cells in 15-20 microliters of LB without antibiotic |
for calcium competent cells. | for calcium competent cells. | ||
</br> | </br> | ||
Revision as of 00:30, 29 September 2011
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MAIN
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PROJECT
20/May/2011 • DNA digestion with SspI and NdeI + Buffer 3 at 37 C , 1 hr. • Electrophoresis 60 V, 1.5 dr. • Pre-inocolum of virgin cells in 15-20 microliters of LB without antibiotic for calcium competent cells. • Purification of DNA of the agarose gel.
