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Team:UT Dallas/notebook dates
From 2011.igem.org
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</div> | </div> | ||
<div id="right"> | <div id="right"> | ||
| - | <h2>Ligation Calculator</h2> | + | <div style="width:720px;margin-bottom: 15px;"><h2>Ligation Calculator</h2></div> |
<div id='july'> <a href='#' onclick="getData('week1');return false;" class="week_link">Week 1 </a> | <div id='july'> <a href='#' onclick="getData('week1');return false;" class="week_link">Week 1 </a> | ||
<br><a href='#' onclick="getData('week2');return false;" class="week_link">Week 2 </a> | <br><a href='#' onclick="getData('week2');return false;" class="week_link">Week 2 </a> | ||
Revision as of 00:24, 29 September 2011
Ligation Calculator
Week 1
| 7/11/2011 | Preparing sent parts |
| 7/12/2011 |
Preparing sent parts growing LB and appropriate antibiotic overnight |
| 7/13/2011 |
all grew, negative controls didn't Preparing sent parts, take 200µL from LB grown overnight and plated on appropriate antibiotic |
| 7/14/2011 |
all grew a blob and a few colonies along the edge Transformation at 37 degrees Celsius overnight- 50 µL DH5a, 2 µL DNA (all carb) Plating sent parts Take one colony from July 13 plate properly |
| 7/15/2011 |
Transformation of 50µL of DH5a, 2 µL DNA (all carb) at 37 degrees overnight Incubated in 3mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm. |
