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Team:ITESM Mexico/Construct
From 2011.igem.org
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[[File:Construct.jpg|500px|center]] | [[File:Construct.jpg|500px|center]] | ||
| - | ::'''Construct''' | + | ===::'''Construct'''=== |
::The construct combining the three previously mentioned plasmids, the green receptor activates the expression of the RecA final product, this RecA protein binds into the operators, allowing the expression of Pbad´s, in presence of arabinose, depending in the concentration of arabinose, if there is a high concentration, the low concentration plasmid is inactivated by the iTast. If there is a low concentration, the plasmid will activate de Pbad´s and the keys and anti-keys to avoid the expression of both fluorescent proteins. | ::The construct combining the three previously mentioned plasmids, the green receptor activates the expression of the RecA final product, this RecA protein binds into the operators, allowing the expression of Pbad´s, in presence of arabinose, depending in the concentration of arabinose, if there is a high concentration, the low concentration plasmid is inactivated by the iTast. If there is a low concentration, the plasmid will activate de Pbad´s and the keys and anti-keys to avoid the expression of both fluorescent proteins. | ||
[[File:High_concentracion.jpg|500px|center]] | [[File:High_concentracion.jpg|500px|center]] | ||
| - | ::'''High concentration Mechanism''' | + | ===::'''High concentration Mechanism'''=== |
::This plasmid works almost as the same as the low concentration mechanism, also the presence of RecA unfolds the loop for PBad, in this case has a low affinity (PBadwk), then also expresses the key (Tawk) for the lock (Crxwk) but the difference in this mechanism is the production of an anti-key (itast) this anti-key prevents the binding of the Tast of the low concentration mechanism preventing the expression of GFP. Also the GFP is actually GFP-LVA tag, this tail allows the rapid degradation of the GFP proteins. | ::This plasmid works almost as the same as the low concentration mechanism, also the presence of RecA unfolds the loop for PBad, in this case has a low affinity (PBadwk), then also expresses the key (Tawk) for the lock (Crxwk) but the difference in this mechanism is the production of an anti-key (itast) this anti-key prevents the binding of the Tast of the low concentration mechanism preventing the expression of GFP. Also the GFP is actually GFP-LVA tag, this tail allows the rapid degradation of the GFP proteins. | ||
[[File:Low_Concentration.jpg|500px|center]] | [[File:Low_Concentration.jpg|500px|center]] | ||
| - | ::'''Low Concentration Mechanism''' | + | ===::'''Low Concentration Mechanism'''=== |
::This Plasmid contains a constitutive promoter for the expression of the lambda repressor which binds into the OR1,OR2 ,OR3 building a loop, this loop avoids the expression of the pBad St, which in presence of arabinose promotes the expression of the next biobricks, the lambda repressor is removed from the operators (OR1, OR2, OR3), when this loops unfolds allows the expression of pBADst, then the expression of Tast is complementary to the Crxst, after binding, allows the expression of the RBS for the expression of GFP. | ::This Plasmid contains a constitutive promoter for the expression of the lambda repressor which binds into the OR1,OR2 ,OR3 building a loop, this loop avoids the expression of the pBad St, which in presence of arabinose promotes the expression of the next biobricks, the lambda repressor is removed from the operators (OR1, OR2, OR3), when this loops unfolds allows the expression of pBADst, then the expression of Tast is complementary to the Crxst, after binding, allows the expression of the RBS for the expression of GFP. | ||
[[File:Photoreceptorplasmid.jpg|500px|center]] | [[File:Photoreceptorplasmid.jpg|500px|center]] | ||
| - | ::'''Photoreceptor''' | + | ===::'''Photoreceptor'''=== |
::The green receptor acts in the presence of a specific wavelength; we see this specific wave as green light. After receiving the green light the plasmid product would be RecA | ::The green receptor acts in the presence of a specific wavelength; we see this specific wave as green light. After receiving the green light the plasmid product would be RecA | ||
Revision as of 00:11, 29 September 2011
ITESM MÉXICO
SensE.coli
Contents |
::Construct
- The construct combining the three previously mentioned plasmids, the green receptor activates the expression of the RecA final product, this RecA protein binds into the operators, allowing the expression of Pbad´s, in presence of arabinose, depending in the concentration of arabinose, if there is a high concentration, the low concentration plasmid is inactivated by the iTast. If there is a low concentration, the plasmid will activate de Pbad´s and the keys and anti-keys to avoid the expression of both fluorescent proteins.
::High concentration Mechanism
- This plasmid works almost as the same as the low concentration mechanism, also the presence of RecA unfolds the loop for PBad, in this case has a low affinity (PBadwk), then also expresses the key (Tawk) for the lock (Crxwk) but the difference in this mechanism is the production of an anti-key (itast) this anti-key prevents the binding of the Tast of the low concentration mechanism preventing the expression of GFP. Also the GFP is actually GFP-LVA tag, this tail allows the rapid degradation of the GFP proteins.
::Low Concentration Mechanism
- This Plasmid contains a constitutive promoter for the expression of the lambda repressor which binds into the OR1,OR2 ,OR3 building a loop, this loop avoids the expression of the pBad St, which in presence of arabinose promotes the expression of the next biobricks, the lambda repressor is removed from the operators (OR1, OR2, OR3), when this loops unfolds allows the expression of pBADst, then the expression of Tast is complementary to the Crxst, after binding, allows the expression of the RBS for the expression of GFP.
::Photoreceptor
- The green receptor acts in the presence of a specific wavelength; we see this specific wave as green light. After receiving the green light the plasmid product would be RecA
