Team:Caltech/Recipes
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==Enrichment Minimal Media== | ==Enrichment Minimal Media== | ||
- | + | For 100 mL media: | |
- | + | ||
* 79 mL Phosphate solution | * 79 mL Phosphate solution | ||
* 10 mL Salt Solution I | * 10 mL Salt Solution I | ||
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* 0.1 mL SL-10 Trace Element Solution. | * 0.1 mL SL-10 Trace Element Solution. | ||
* variable amounts of EDC's | * variable amounts of EDC's | ||
+ | |||
+ | Phosphate solution: 1.0712g K2 HPO4 and 0.5239g KH2 PO4 per liter of water<br/> | ||
+ | Salt Solution I: 3g NH4Cl, 10g NaCl, 5g KCl, 1.5g Na2SO4 per liter of water<br/> | ||
+ | Salt Solution II: 4g MgCl2.6H20 and 0.5g CaCl2.2H20 per liter of water<br/> | ||
+ | Wolfe's Vitamin Solution (100x): 10 mg Pyridoxine.Hcl, 5 mg p-Aminobenzoic acid, 5 mg Lipoic acid, 5 mg Nicotinic acid, 5 mg Riboflavin, 5 mg Thiamine.HCl, 5 mg Pantothenic acid, 2 mg Biotin, 2 mg Folic acid, 0.1 mg Vitamin B12 per liter of water. <br/> | ||
+ | Trace Element Solution: 1500 mg FeCl2.4H2O, 70 mg ZnCl2, 100 mg MnCl2.4H2O, 6 mg H3BO3, 190 mg CoCl2.6H2O, 2 mg CuCl2.2H2O, 24 mg NiCl2.6H2O, 31 mg Na2MoO4 per liter of water<br/> | ||
Latest revision as of 00:00, 29 September 2011
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50% glycerol Stock:
Agar/LB plate (Autoclaved):
Ampicillin Stock
Chloramphenicol Stock
Enrichment Minimal MediaFor 100 mL media:
Phosphate solution: 1.0712g K2 HPO4 and 0.5239g KH2 PO4 per liter of water
Gibson Mix (1.33x)For 25 aliquots of 15 μl each:
IPTG stockFor 1000x stock (.1M)
Ligation ReactionFor a 20 uL reaction:
p450 degradation testing solutionFor a 200uL reaction:
Phusion PCRFor a 50uL reaction:
SOC Mediafor 250 ml (adapted from [http://openwetware.org/wiki/SOC OpenWetWare]):
Restriction DigestFor a 50uL reaction:
Taq PCR (for 16s insert)For a 25uL reaction:
X-gal stock (50x)For 20mg/mL, total 0.5mL volume:
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