Team:UANL Mty-Mexico/Contributions/Parts

From 2011.igem.org

(Difference between revisions)
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{{:Team:UANL_Mty-Mexico/Templates/Banner-blueprints}}
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{{:Team:UANL_Mty-Mexico/Templates/Banner-template}}
{{:Team:UANL_Mty-Mexico/Templates/Menu-template}}
{{:Team:UANL_Mty-Mexico/Templates/Menu-template}}
<html>
<html>
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<META NAME="robots" CONTENT="FOLLOW,INDEX">
<META NAME="robots" CONTENT="FOLLOW,INDEX">
    
    
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<link href="http://www.genobiotec2011.org/iGEMwiki/mainStyle.css" rel="stylesheet" type="text/css">
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<script type="text/javascript" src="http://www.genobiotec2011.org/iGEMwiki/Scripts/jquery-1.3.2.min.js"></script>
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<script type="text/javascript" src="http://www.genobiotec2011.org/iGEMwiki/Scripts/jquery-1.3.2.min.js"></script>
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<script type='text/javascript' src='http://www.genobiotec2011.org/iGEMwiki/jquery.easing.1.3.js'></script>
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<script type='text/javascript' src='http://www.genobiotec2011.org/iGEMwiki/jquery.slideup.menu.1.0.min.js'></script>
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<script type="text/javascript" src="http://www.genobiotec2011.org/iGEMwiki/Scripts/jquery.scroll-follow.js"></script>
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<script type="text/javascript" src="http://www.genobiotec2011.org/iGEMwiki/Scripts/slimbox2/js/slimbox2.js"></script>
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<script>
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    var opts = {slideUpSpeed:500, slideDownSpeed: 200};
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    $(document).ready(function(){
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$('.top-menu').removeClass().addClass('top-menu').addClass('style5');
 +
      $('ul.s-menu li').click(function(){
 +
        $(this).parent().find('li').removeClass('selected');
 +
        $(this).addClass('selected');
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      });
 +
      $('ul.s-style li').click(function(){
 +
      $('.top-menu').removeClass().addClass('top-menu').addClass('style'+$(this).attr('rel'));
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      $('.top-menu').removeClass().addClass('top-menu').addClass('style5');
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 +
        $(".top-menu").slideupmenu(opts); 
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      });
 +
     
 +
      $('ul.s-way li[rel="1"]').live('click', function() {
 +
        opts = {slideUpSpeed:500, slideDownSpeed: 200};
 +
        $(".top-menu").slideupmenu(opts); 
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      });
 +
      $('ul.s-way li[rel="2"]').live('click', function() {
 +
        opts = {slideUpSpeed: 500, slideDownSpeed: 500, ease: "easeOutBounce", stopQueue: true};
 +
        $(".top-menu").slideupmenu(opts); 
 +
      });
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      $('ul.s-way li[rel="3"]').live('click', function() {
 +
        opts = {slideUpSpeed: 1000, slideDownSpeed: 100, ease: "easeOutBack", stopQueue: true};
 +
        $(".top-menu").slideupmenu(opts); 
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      });
 +
      $('ul.s-way li[rel="4"]').live('click', function() {
 +
        opts = {slideUpSpeed: 500, slideDownSpeed: 500, ease:false, stopQueue: false};
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        $(".top-menu").slideupmenu(opts); 
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      });
 +
      $(".top-menu").slideupmenu(opts); 
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    });
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  </script>
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  <script type="text/javascript">
 +
  $( document ).ready( function ()
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{
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$( '#rightColumn' ).scrollFollow();
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}
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);
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  </script>
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</head>
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{{:Team:UANL_Mty-Mexico/Templates/Banner-template}}
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{{:Team:UANL_Mty-Mexico/Templates/Menu-template}}
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<html>
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<head>
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<!--
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============================================================================================
 +
*** Acknowledgments ***
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 +
>>>To TU_Delf Team
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 +
Thank you so much to TU_Delft iGEM2010 team for providing us with so many examples of html code and how to apply them.
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 +
If you like some of this code please check it out in their wiki: https://2010.igem.org/Team:TU_Delft#page=Modeling/wiki-tips-tricks
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============================================================================================
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/-->
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<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
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  <title>Team: UANL_Mty-Mexico</title>
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<META NAME="keywords" CONTENT="UANL,iGEM,Universidad,Autonoma,Nuevo,Leon,Biphasic,switch,Lambda,Genetic,Regulation,Quorum,Sensing,pRM,OL,Green Light,Red Light">
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<META NAME="description" CONTENT="iGEM-UANL is the representative team from Universidad Autonoma de Nuevo León, at Monterrey, México. This team is composed of ten students who spent their summer in the lab, having fun with transformations, constructions and plasmidic DNA extractions. This">
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<META NAME="abstract" CONTENT="Information processing through living things remains a challenge to science. Genetic logic-gates and">
 +
<META NAME="author" CONTENT="iGEM-UANL">
 +
<META NAME="robots" CONTENT="FOLLOW,INDEX">
 +
 
<link href="http://www.genobiotec2011.org/iGEMwiki/mainStyle.css" rel="stylesheet" type="text/css">
<link href="http://www.genobiotec2011.org/iGEMwiki/mainStyle.css" rel="stylesheet" type="text/css">
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<script type="text/javascript" src="http://www.genobiotec2011.org/iGEMwiki/Scripts/slimbox2/js/slimbox2.js"></script>
<script type="text/javascript" src="http://www.genobiotec2011.org/iGEMwiki/Scripts/slimbox2/js/slimbox2.js"></script>
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);
);
   </script>  
   </script>  
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</head>
</head>
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<body>
<body>
 +
<div class="content">
<div class="content">
   <div class="main" style="width:930px">
   <div class="main" style="width:930px">
   <!--<div style="width:900px">-->
   <!--<div style="width:900px">-->
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    <div class="br2"></div><div class="br2"></div><div class="br2"></div>           
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     <div id="leftColumn">
     <div id="leftColumn">
     <div id="ColorHeader">
     <div id="ColorHeader">
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             Contributions: Parts
             Contributions: Parts
     </div>
     </div>
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<div class="br"></div><div class="br"></div>
<div class="br"></div><div class="br"></div>
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<center>
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<table width="700px">
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<table width="650px">
   <tr class="yellow">  
   <tr class="yellow">  
     <td class="adjacent">Part name</td>
     <td class="adjacent">Part name</td>
     <td class="adjacent">Description</td>
     <td class="adjacent">Description</td>
 +
    <td class="adjacent">Favorited</td>
   </tr>
   </tr>
   <tr>
   <tr>
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     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566000">BBa_K566000</a></td>
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     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566000" target="_new">BBa_K566000</a></td>
-
     <td class="final"><b>OL region from Lambda phage</b> allows biphasic switch <a href="http://partsregistry.org/Part:BBa_K566002" >(K566002)</a> effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter <a href="http://partsregistry.org/Part:BBa_K566001" >(K566001)</a>.</td>
+
     <td class="final"><b>OL region from Lambda phage</b> allows biphasic switch <a href="http://partsregistry.org/Part:BBa_K566002" target="_new" >(K566002)</a> effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter <a href="http://partsregistry.org/Part:BBa_K566001" target="_new" >(K566001)</a>.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566001">BBa_K566001</a></td>
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     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566001" target="_new">BBa_K566001</a></td>
-
     <td class="final"><b>pRM promoter from Lambda</b> phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] induce it, high [cI] repress it. It needs OL region <a href="http://partsregistry.org/Part:BBa_K566000" >(K566000)</a> for effective repression. Makes the biphasic switch <a href="http://partsregistry.org/Part:BBa_K566002" >(K566002)</a> together with OL region. </td>
+
     <td class="final"><b>pRM promoter from Lambda</b> phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] induce it, high [cI] repress it. It needs OL region <a href="http://partsregistry.org/Part:BBa_K566000" target="_new" >(K566000)</a> for effective repression. Makes the biphasic switch <a href="http://partsregistry.org/Part:BBa_K566002" >(K566002)</a> together with OL region. </td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566002">BBa_K566002</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566002" target="_new">BBa_K566002</a></td>
     <td class="final">The <b>Biphasic Switch</b> combines positive and negative regulation through a single input. It is turned ON by low lambda cI concentrations and OFF by high cI concentrations.</td>
     <td class="final">The <b>Biphasic Switch</b> combines positive and negative regulation through a single input. It is turned ON by low lambda cI concentrations and OFF by high cI concentrations.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566003">BBa_K566003</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566003" target="_new">BBa_K566003</a></td>
-
     <td class="final"> <b>CFP optimized for <i>E. coli</i>.</b> Cyan fluorescent protein optimized from part <a href="http://partsregistry.org/Part:BBa_E0022">BBa_E0022</a> with preferential codon usage for improved expression in <i>E. coli</i>. It is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea victoria . It possesses bright fluorescence with exitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1.</td>
+
     <td class="final"> <b>CFP optimized for <i>E. coli</i>.</b> Cyan fluorescent protein optimized from part <a href="http://partsregistry.org/Part:BBa_E0022" target="_new">BBa_E0022</a> with preferential codon usage for improved expression in <i>E. coli</i>. It is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea victoria . It possesses bright fluorescence with exitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566004">BBa_K566004</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566004" target="_new">BBa_K566004</a></td>
-
     <td class="final"><b>Protein generator of cyan fluorescent protein without transcription terminator.</b> It may be negatively regulated by Mnt repressor <a href="http://partsregistry.org/Part:BBa_C0072">(BBa_C0072)</a>.</td>
+
     <td class="final"><b>Protein generator of cyan fluorescent protein without transcription terminator.</b> It may be negatively regulated by Mnt repressor <a href="http://partsregistry.org/Part:BBa_C0072" target="_new">(BBa_C0072)</a>.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566005">BBa_K566005</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566005" target="_new">BBa_K566005</a></td>
     <td class="final"><b>pCpcG2 promoter (Green light inducible).</b> Green light inducible pCpcG2 promoter from pJT122 plasmid constructed by Tabor et al. (2010). It is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light. Light intensities must be carefully regulated to achieve successful gene expression.</td>
     <td class="final"><b>pCpcG2 promoter (Green light inducible).</b> Green light inducible pCpcG2 promoter from pJT122 plasmid constructed by Tabor et al. (2010). It is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light. Light intensities must be carefully regulated to achieve successful gene expression.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566006">BBa_K566006</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566006" target="_new">BBa_K566006</a></td>
     <td class="final"> <b>penI-pCpcG2-pPenI-mnt.</b> Repression-based potential AND gate. Mnt Repressor is under the control of the PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter. pCpcG2 promoter is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light.</td>
     <td class="final"> <b>penI-pCpcG2-pPenI-mnt.</b> Repression-based potential AND gate. Mnt Repressor is under the control of the PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter. pCpcG2 promoter is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566007">BBa_K566007</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566007" target="_new">BBa_K566007</a></td>
-
     <td class="final"><b>PenI repressor optimized for E. coli (inverted sequence).</b> Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in <i>E. coli</i>. PenI negatively regulates transcription from pPenI promoter <a href="http://partsregistry.org/Part:BBa_R0074">(BBa_R0074)</a>.</td>
+
     <td class="final"><b>PenI repressor optimized for E. coli (inverted sequence).</b> Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in <i>E. coli</i>. PenI negatively regulates transcription from pPenI promoter <a href="http://partsregistry.org/Part:BBa_R0074" target="_new">(BBa_R0074)</a>.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566008">BBa_K566008</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566008" target="_new">BBa_K566008</a></td>
-
     <td class="final"><b>Mnt repressor optimized for <i>E. coli</i>.<b> Mnt repressor optimized from part BBa_C0072 with preferential codon usage for improved expression in <i>E. coli</i>. It acts on pMnt promoter <a href="http://partsregistry.org/Part:BBa_R0073">(BBa_R0073)</a>.</td>
+
     <td class="final"><b>Mnt repressor optimized for <i>E. coli</i>.<b> Mnt repressor optimized from part BBa_C0072 with preferential codon usage for improved expression in <i>E. coli</i>. It acts on pMnt promoter <a href="http://partsregistry.org/Part:BBa_R0073" target="_new">(BBa_R0073)</a>.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566009">BBa_K566009</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566009" target="_new">BBa_K566009</a></td>
     <td class="final"><b>pCpcG2 promoter, inverted sequence (Green light inducible).</b> Inverted Green light inducible pCpcG2 promoter, from pJT122 plasmid constructed by Tabor et al. (2010). It is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light. Light intensities must be carefully regulated to achieve successful gene expression. The sequence was inverted to ease DNA synthesis.</td>
     <td class="final"><b>pCpcG2 promoter, inverted sequence (Green light inducible).</b> Inverted Green light inducible pCpcG2 promoter, from pJT122 plasmid constructed by Tabor et al. (2010). It is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light. Light intensities must be carefully regulated to achieve successful gene expression. The sequence was inverted to ease DNA synthesis.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
    
    
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566010">BBa_K566010</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566010" target="_new">BBa_K566010</a></td>
-
     <td class="final"><b>PenI repressor optimized for E. coli (inverted sequence).</b> Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in <i>E. coli</i>. Negatively regulates transcription from pPenI promoter. <a href="http://partsregistry.org/Part:BBa_R0074">(BBa_R0074)</a>. The nucleotide sequence was inverted to ease DNA synthesis.</td>
+
     <td class="final"><b>PenI repressor optimized for E. coli (inverted sequence).</b> Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in <i>E. coli</i>. Negatively regulates transcription from pPenI promoter. <a href="http://partsregistry.org/Part:BBa_R0074" target="_new">(BBa_R0074)</a>. The nucleotide sequence was inverted to ease DNA synthesis.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>  
   </tr>  
    
    
   <tr>
   <tr>
   
   
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566011">BBa_K566011</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566011" target="_new">BBa_K566011</a></td>
     <td class="final"><b>cI434 repressor from phage 434 optimized for E. coli cI (+LVA).</b> Optimized cI434 repressor with LVA tag from phage 434, with preferential codon usage for improved expression in <i>E. coli</i>.</td>
     <td class="final"><b>cI434 repressor from phage 434 optimized for E. coli cI (+LVA).</b> Optimized cI434 repressor with LVA tag from phage 434, with preferential codon usage for improved expression in <i>E. coli</i>.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>  
   </tr>  
    
    
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566012">BBa_K566012</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566012" target="_new">BBa_K566012</a></td>
     <td class="final"><b>cI repressor from Lambda phage optimized for <i>E. coli</i>.</b> Includes LVA tag for faster degradation.<br/>
     <td class="final"><b>cI repressor from Lambda phage optimized for <i>E. coli</i>.</b> Includes LVA tag for faster degradation.<br/>
It has preferential codons to improve their expression in <i>E. coli</i>.</td>
It has preferential codons to improve their expression in <i>E. coli</i>.</td>
 +
<td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>   
   </tr>   
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566013">BBa_K566013</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566013" target="_new">BBa_K566013</a></td>
     <td class="final"><b>RFP optimized for <i>E. coli</i> (+LVA).</b> Includes LVA tag for faster degradation.<br/>
     <td class="final"><b>RFP optimized for <i>E. coli</i> (+LVA).</b> Includes LVA tag for faster degradation.<br/>
Preferential codons usage to improve their expression in <i>E. coli</i>.</td>
Preferential codons usage to improve their expression in <i>E. coli</i>.</td>
 +
<td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>   
   </tr>   
    
    
    
    
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566015">BBa_K566015</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566015" target="_new">BBa_K566015</a></td>
     <td class="final"><b>pRM-RBS-cI434.</b> Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required. <br/>
     <td class="final"><b>pRM-RBS-cI434.</b> Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required. <br/>
-
A RBS sequence from the Elowitz repressilator that defines the RBS efficiency <a href="http://partsregistry.org/Part:BBa_B0034">(BBa_B0034)</a>. Also contain the cI434 coding sequence optimized for <i>E. coli</i>, its come from the registered part cI434 repressor protein <a href="http://partsregistry.org/Part:BBa_C0052">(BBa_C0052)</a>, It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation. </td>
+
A RBS sequence from the Elowitz repressilator that defines the RBS efficiency <a href="http://partsregistry.org/Part:BBa_B0034" target="_new">(BBa_B0034)</a>. Also contain the cI434 coding sequence optimized for <i>E. coli</i>, its come from the registered part cI434 repressor protein <a href="http://partsregistry.org/Part:BBa_C0052" target="_new">(BBa_C0052)</a>, It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation. </td>
 +
<td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>   
   </tr>   
    
    
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566016" >BBa_K566016</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566016" target="_new" >BBa_K566016</a></td>
     <td class="final"> <b>OL-pTetMnt-cI.</b> Induction system for Biphasic Switch. <br/>
     <td class="final"> <b>OL-pTetMnt-cI.</b> Induction system for Biphasic Switch. <br/>
It responds to negative repression from TetR or cI repressor. </td>
It responds to negative repression from TetR or cI repressor. </td>
 +
<td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>  
   </tr>  
    
    
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566017" >BBa_K566017</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566017" target="_new" >BBa_K566017</a></td>
     <td class="final"><b>pRM434-RFP.</b> Red Fluorescent Protein under the control of pRM434 promoter. It was constructed for demonstrating dual state of a biphasic switch. </td>
     <td class="final"><b>pRM434-RFP.</b> Red Fluorescent Protein under the control of pRM434 promoter. It was constructed for demonstrating dual state of a biphasic switch. </td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>  
   </tr>  
    
    
Line 217: Line 335:
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566019">BBa_K566019</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566019" target="_new">BBa_K566019</a></td>
     <td class="final"><b>E. coli MxRed.</b> Derived from Dr. Jeff Tabor JT2 <i>E. coli</i> strain, this part is a built-in red-light induction system in <i>E. coli</i> made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher. <br/>
     <td class="final"><b>E. coli MxRed.</b> Derived from Dr. Jeff Tabor JT2 <i>E. coli</i> strain, this part is a built-in red-light induction system in <i>E. coli</i> made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher. <br/>
It is proposed as a photo-chassis that could make useful tools in this field of light induction. <br/>
It is proposed as a photo-chassis that could make useful tools in this field of light induction. <br/>
Integration include the genes pcyA, ho1, Cph8 and a Mnt working as follows: Genes ho1 and pcyA are responsible for the chromophore synthesis. Cph8 codes for the chimaeric red-light receptor. These three genes are constitutively expressed. Mnt repressor is expressed from pOmpC promoter, and the red light stops its induction. It is therefore used as a NOT-gate to regulate expression from pMnt.
Integration include the genes pcyA, ho1, Cph8 and a Mnt working as follows: Genes ho1 and pcyA are responsible for the chromophore synthesis. Cph8 codes for the chimaeric red-light receptor. These three genes are constitutively expressed. Mnt repressor is expressed from pOmpC promoter, and the red light stops its induction. It is therefore used as a NOT-gate to regulate expression from pMnt.
  </td>
  </td>
 +
  <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
    
    
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566020">BBa_K566020</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566020" target="_new">BBa_K566020</a></td>
     <td class="final"><b>Mnt repressor regulated by pOmpC.</b> </td>
     <td class="final"><b>Mnt repressor regulated by pOmpC.</b> </td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
    
    
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566021">BBa_K566021</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566021" target="_new">BBa_K566021</a></td>
     <td class="final"><b>pcyA CDS.</b></td>
     <td class="final"><b>pcyA CDS.</b></td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566022" >BBa_K566022</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566022" target="_new" >BBa_K566022</a></td>
     <td class="final"><b>ho1 CDS</b></td>
     <td class="final"><b>ho1 CDS</b></td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
    
    
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566023" >BBa_K566023</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566023" target="_new">BBa_K566023</a></td>
     <td class="final"><b>pcyA gene (constitutive expression).</b> pcyA gene transcribed from constitutive promoter.</td>
     <td class="final"><b>pcyA gene (constitutive expression).</b> pcyA gene transcribed from constitutive promoter.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
    
    
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566024" >BBa_K566024</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566024" target="_new" >BBa_K566024</a></td>
     <td class="final"><b>ho1 gene (constitutive expression).</b> ho1 gene transcribed from constitutive promoter. ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB). </td>
     <td class="final"><b>ho1 gene (constitutive expression).</b> ho1 gene transcribed from constitutive promoter. ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB). </td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
Line 256: Line 380:
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566025" >BBa_K566025</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566025" target="_new" >BBa_K566025</a></td>
     <td class="final"><b>Cph8 CDS.</b> The red light-sensing protein Cph8 is expressed in the phosphorylated ground state. It is switched to the unphosphorylated state by 650-nm light and back to the phosphorylated state by 705-nm light. When phosphorylated, Cph8 passes a phosphoryl group to OmpR, which then binds to and activates transcription from PompC. Because it is inactivated by red light, Cph8 can be considered a logical (NOT red) sensor. A genetic inverter or logical NOT gate is used to invert the response of the (NOT red) sensor to that of a red light sensor.</td>
     <td class="final"><b>Cph8 CDS.</b> The red light-sensing protein Cph8 is expressed in the phosphorylated ground state. It is switched to the unphosphorylated state by 650-nm light and back to the phosphorylated state by 705-nm light. When phosphorylated, Cph8 passes a phosphoryl group to OmpR, which then binds to and activates transcription from PompC. Because it is inactivated by red light, Cph8 can be considered a logical (NOT red) sensor. A genetic inverter or logical NOT gate is used to invert the response of the (NOT red) sensor to that of a red light sensor.</td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
Line 263: Line 388:
   <tr>
   <tr>
-
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566026" >BBa_K566026</a></td>
+
     <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566026" target="_new" >BBa_K566026</a></td>
     <td class="final"><b>Cph8 gene for red-photoreceptor (constitutive expression).</b> Cassette for constitutive expression of Cph8, originally constructed by Dr. Jeff Tabor.<br/>
     <td class="final"><b>Cph8 gene for red-photoreceptor (constitutive expression).</b> Cassette for constitutive expression of Cph8, originally constructed by Dr. Jeff Tabor.<br/>
This gene codifies for the red light-sensing protein Cph8, which is expressed in the phosphorylated ground state. It is switched to the unphosphorylated state by 650-nm light and back to the phosphorylated state by 705-nm light. When phosphorylated, Cph8 passes a phosphoryl group to OmpR, which then binds to and activates transcription from PompC. Because it is inactivated by red light, Cph8 can be considered a logical (NOT red) sensor. A genetic inverter or logical NOT gate is used to invert the response of the (NOT red) sensor to that of a red light sensor. </td>
This gene codifies for the red light-sensing protein Cph8, which is expressed in the phosphorylated ground state. It is switched to the unphosphorylated state by 650-nm light and back to the phosphorylated state by 705-nm light. When phosphorylated, Cph8 passes a phosphoryl group to OmpR, which then binds to and activates transcription from PompC. Because it is inactivated by red light, Cph8 can be considered a logical (NOT red) sensor. A genetic inverter or logical NOT gate is used to invert the response of the (NOT red) sensor to that of a red light sensor. </td>
 +
<td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
   </tr>
   </tr>
 +
    <tr>
 +
    <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566027" target="_new">BBa_K566027</a></td>
 +
    <td class="final"><b>ho1 CDS</b></td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
 +
  </tr>
 +
 
 +
    <tr>
 +
    <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566028" target="_new" >BBa_K566028</a></td>
 +
    <td class="final"><b>ho1 CDS</b></td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
 +
  </tr>
 +
 
 +
    <tr>
 +
    <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566029" target="_new" >BBa_K566029</a></td>
 +
    <td class="final"><b>ho1 CDS</b></td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
 +
  </tr>
 +
 
 +
    <tr>
 +
    <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566030" target="_new" >BBa_K566030</a></td>
 +
    <td class="final"><b>ho1 CDS</b></td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
 +
  </tr>
 +
 
 +
    <tr>
 +
    <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566031" target="_new" >BBa_K566031</a></td>
 +
    <td class="final"><b>ho1 CDS</b></td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
 +
  </tr>
 +
 
 +
    <tr>
 +
    <td class="adjacent"><a href="http://partsregistry.org/Part:BBa_K566032" target="_new" >BBa_K566032</a></td>
 +
    <td class="final"><b>ho1 CDS</b></td>
 +
    <td class="adjacent"><img src="https://static.igem.org/mediawiki/2011/2/2e/Star.png "width="20px" height="20px" alt="star" align="center"></td>
 +
  </tr>
 +
 
</table>
</table>
 +
</center>
</div>
</div>
      
      

Revision as of 23:48, 28 September 2011

banner-main iGEM-logo
Team: UANL_Mty-Mexico Team: UANL_Mty-Mexico {{:Team:UANL_Mty-Mexico/Templates/Banner-template}} {{:Team:UANL_Mty-Mexico/Templates/Menu-template}} Team: UANL_Mty-Mexico
Contributions: Parts
Parts
Part name Description Favorited
BBa_K566000 OL region from Lambda phage allows biphasic switch (K566002) effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter (K566001). star
BBa_K566001 pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] induce it, high [cI] repress it. It needs OL region (K566000) for effective repression. Makes the biphasic switch (K566002) together with OL region. star
BBa_K566002 The Biphasic Switch combines positive and negative regulation through a single input. It is turned ON by low lambda cI concentrations and OFF by high cI concentrations. star
BBa_K566003 CFP optimized for E. coli. Cyan fluorescent protein optimized from part BBa_E0022 with preferential codon usage for improved expression in E. coli. It is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea victoria . It possesses bright fluorescence with exitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1. star
BBa_K566004 Protein generator of cyan fluorescent protein without transcription terminator. It may be negatively regulated by Mnt repressor (BBa_C0072). star
BBa_K566005 pCpcG2 promoter (Green light inducible). Green light inducible pCpcG2 promoter from pJT122 plasmid constructed by Tabor et al. (2010). It is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light. Light intensities must be carefully regulated to achieve successful gene expression. star
BBa_K566006 penI-pCpcG2-pPenI-mnt. Repression-based potential AND gate. Mnt Repressor is under the control of the PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter. pCpcG2 promoter is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light. star
BBa_K566007 PenI repressor optimized for E. coli (inverted sequence). Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in E. coli. PenI negatively regulates transcription from pPenI promoter (BBa_R0074). star
BBa_K566008 Mnt repressor optimized for E. coli. Mnt repressor optimized from part BBa_C0072 with preferential codon usage for improved expression in E. coli. It acts on pMnt promoter (BBa_R0073). star
BBa_K566009 pCpcG2 promoter, inverted sequence (Green light inducible). Inverted Green light inducible pCpcG2 promoter, from pJT122 plasmid constructed by Tabor et al. (2010). It is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light. Light intensities must be carefully regulated to achieve successful gene expression. The sequence was inverted to ease DNA synthesis. star
BBa_K566010 PenI repressor optimized for E. coli (inverted sequence). Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in E. coli. Negatively regulates transcription from pPenI promoter. (BBa_R0074). The nucleotide sequence was inverted to ease DNA synthesis. star
BBa_K566011 cI434 repressor from phage 434 optimized for E. coli cI (+LVA). Optimized cI434 repressor with LVA tag from phage 434, with preferential codon usage for improved expression in E. coli. star
BBa_K566012 cI repressor from Lambda phage optimized for E. coli. Includes LVA tag for faster degradation.
It has preferential codons to improve their expression in E. coli.
star
BBa_K566013 RFP optimized for E. coli (+LVA). Includes LVA tag for faster degradation.
Preferential codons usage to improve their expression in E. coli.
star
BBa_K566015 pRM-RBS-cI434. Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required.
A RBS sequence from the Elowitz repressilator that defines the RBS efficiency (BBa_B0034). Also contain the cI434 coding sequence optimized for E. coli, its come from the registered part cI434 repressor protein (BBa_C0052), It binds to the 434 regulatory sequence in λ pRM promoter. The sequence contains a LVA tag for faster degradation.
star
BBa_K566016 OL-pTetMnt-cI. Induction system for Biphasic Switch.
It responds to negative repression from TetR or cI repressor.
star
BBa_K566017 pRM434-RFP. Red Fluorescent Protein under the control of pRM434 promoter. It was constructed for demonstrating dual state of a biphasic switch. star
BBa_K566019 E. coli MxRed. Derived from Dr. Jeff Tabor JT2 E. coli strain, this part is a built-in red-light induction system in E. coli made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher.
It is proposed as a photo-chassis that could make useful tools in this field of light induction.
Integration include the genes pcyA, ho1, Cph8 and a Mnt working as follows: Genes ho1 and pcyA are responsible for the chromophore synthesis. Cph8 codes for the chimaeric red-light receptor. These three genes are constitutively expressed. Mnt repressor is expressed from pOmpC promoter, and the red light stops its induction. It is therefore used as a NOT-gate to regulate expression from pMnt.
star
BBa_K566020 Mnt repressor regulated by pOmpC. star
BBa_K566021 pcyA CDS. star
BBa_K566022 ho1 CDS star
BBa_K566023 pcyA gene (constitutive expression). pcyA gene transcribed from constitutive promoter. star
BBa_K566024 ho1 gene (constitutive expression). ho1 gene transcribed from constitutive promoter. ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB). star
BBa_K566025 Cph8 CDS. The red light-sensing protein Cph8 is expressed in the phosphorylated ground state. It is switched to the unphosphorylated state by 650-nm light and back to the phosphorylated state by 705-nm light. When phosphorylated, Cph8 passes a phosphoryl group to OmpR, which then binds to and activates transcription from PompC. Because it is inactivated by red light, Cph8 can be considered a logical (NOT red) sensor. A genetic inverter or logical NOT gate is used to invert the response of the (NOT red) sensor to that of a red light sensor. star
BBa_K566026 Cph8 gene for red-photoreceptor (constitutive expression). Cassette for constitutive expression of Cph8, originally constructed by Dr. Jeff Tabor.
This gene codifies for the red light-sensing protein Cph8, which is expressed in the phosphorylated ground state. It is switched to the unphosphorylated state by 650-nm light and back to the phosphorylated state by 705-nm light. When phosphorylated, Cph8 passes a phosphoryl group to OmpR, which then binds to and activates transcription from PompC. Because it is inactivated by red light, Cph8 can be considered a logical (NOT red) sensor. A genetic inverter or logical NOT gate is used to invert the response of the (NOT red) sensor to that of a red light sensor.
star
BBa_K566027 ho1 CDS star
BBa_K566028 ho1 CDS star
BBa_K566029 ho1 CDS star
BBa_K566030 ho1 CDS star
BBa_K566031 ho1 CDS star
BBa_K566032 ho1 CDS star
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Team: UANL_Mty-Mexico