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Team:Wisconsin-Madison/alkane
From 2011.igem.org
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| - | In the production of large chain alkanes for biofuel production, it is crucial for there to be a rapid and accurate diagnostic for comparing production rates in engineered strains of <i>E. coli.</i> To develop an alkane biosensor, <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a> from <i>Pseudomonas putida</i> and <i>Alcanivorax borkumensis</i> were isolated and constructed into a pair of <a href="https://2011.igem.org/Team:Wisconsin-Madison/plasmids">plasmids</a> which code for proteins that bind to alkanes | + | In the production of large chain alkanes for biofuel production, it is crucial for there to be a rapid and accurate diagnostic for comparing production rates in engineered strains of <i>E. coli.</i> To develop an alkane biosensor, <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a> from <i>Pseudomonas putida</i> and <i>Alcanivorax borkumensis</i> were isolated and constructed into a pair of <a href="https://2011.igem.org/Team:Wisconsin-Madison/plasmids">plasmids</a> which code for proteins that bind to alkanes. This induces a promoter upstream of a red fluorescent protein, which can be detected easily using various methods. Similar to our <a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">ethanol sensor</a>, we can find a linear regression by gathering data at different concentrations of n-alkanes, allowing for the quantification of an unknown alkane concentration in a media based upon the level of fluorescence. |
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Learn more about: <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a>, <a href="https://2011.igem.org/Team:Wisconsin-Madison/plasmid">plasmids</a>. | Learn more about: <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a>, <a href="https://2011.igem.org/Team:Wisconsin-Madison/plasmid">plasmids</a>. | ||
Revision as of 23:44, 28 September 2011
Project >>
Overview,
Ethanol Sensor,
Alkane Sensor,
Microcompartment
Alkane Sensor
In the production of large chain alkanes for biofuel production, it is crucial for there to be a rapid and accurate diagnostic for comparing production rates in engineered strains of E. coli. To develop an alkane biosensor, genes from Pseudomonas putida and Alcanivorax borkumensis were isolated and constructed into a pair of plasmids which code for proteins that bind to alkanes. This induces a promoter upstream of a red fluorescent protein, which can be detected easily using various methods. Similar to our ethanol sensor, we can find a linear regression by gathering data at different concentrations of n-alkanes, allowing for the quantification of an unknown alkane concentration in a media based upon the level of fluorescence. |
