Team:Caltech/Recipes
From 2011.igem.org
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==Chloramphenicol Stock== | ==Chloramphenicol Stock== | ||
- | * | + | *For 25 mg/ml stock solution (usually, chloramphenicol is used at 12.5 µg/ml or 25 µg/ml, add 250 mg chloramphenicol to 10 ml 100% ethanol |
- | + | *Store at -20°C | |
- | * | + | |
Revision as of 23:04, 28 September 2011
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50% glycerol Stock:
Agar/LB plate (Autoclaved):
Ampicillin Stock
Chloramphenicol Stock
Enrichment Minimal Media
Gibson Mix (1.33x)For 25 aliquots of 15 μl each:
IPTG stockFor 1000x stock (.1M)
Ligation Reactionfor 20 uL rxn
H&subO make up to 20 uL Total of between 50 and 100ng DNA p450 degradation testing solutionFor a 200uL reaction:
Phusion PCRFor a 50uL reaction:
SOC Mediafor 250 ml (adapted from [http://openwetware.org/wiki/SOC OpenWetWare])
Restriction Digestfor 50uL rxn
Taq PCR (for 16s insert)For a 25uL reaction:
X-gal stock (50x)For 20mg/mL, total 0.5mL volume:
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