"

From 2011.igem.org

Revision as of 12:18, 28 September 2011 (view source) Rodrigo (Talk | contribs) ← Older edit		Revision as of 22:26, 28 September 2011 (view source) Lau (Talk | contribs) Newer edit →
Line 1:		Line 1:
	{{Team:ITESM_Mexico/Top}}		{{Team:ITESM_Mexico/Top}}
-	Some aditional stuff.
-	[[File:Giant_microbe.png|center]]
-	Home made quick saliva DNA extraction protocol:	+	::::::'''Some aditional stuff.''''
		+
		+	[[File:Giant_microbe.png|center]]
-	• Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass	+	:::*''Home made quick saliva DNA extraction protocol:''
-	• Prepare a solution with soap and water in a beaker	+	::#Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass
		+	::#Prepare a solution with soap and water in a beaker
		+	::#Add the soap solution to the last rinse
		+	::#Add frozen (0°C) etanol  to the last rinse
		+	::#Observe how a DNA thread forms, take it and place it in an eppendorf tube.
-	• Add the soap solution to the last rinse
-	• Add frozen (0°C) etanol  to the last rinse	+	:::*''Abbreviations and glossary:''
-	• Observe how a DNA thread forms, take it and place it in an eppendorf tube.	+	::*'''Biobrickoatl:'''It's the biobrick deity for the Mesoamerican cultures, although at that time they didn't knew what a biobrick meant. The great feathered biobrick serpent vigilates over all new and old biobricks.
		+	::*'''Competent cells:''' Cells that can incorporate, in an easier way, foreign DNA into their cell walls.
		+	::*'''CcaS-EnvZ: '''Is the green light receptor that induces the expresión of phycobilisome linker protein.
		+	::*'''CFP:''' Cyan Fluorescent Protein
		+	::*'''Crst:''' Lock for the PbadST (strong, for low concentrations) promoter
		+	::*'''CrWk:''' Lock for the Pbad WK (weak, for high concentration) promoter
		+	::*'''GFP:''' Green Fluorescent Protein
		+	::*'''ho1:''' Heme oxigenase 1
		+	::*'''iTaST:''' Inverse TaST, complementary sequence to the Key TaST, blocks TaST activity
		+	::*'''pBADST:''' Strong arabinose promoter for low concentration detection.
		+	::*'''pBADWk:''' Weak arabinose promoter for high concentration detection.
		+	::*'''Pcy4A:''' Phycocyanobilin ferredoxin oxidoreductase.
		+	::*'''RBS:''' Ribosome binding site
		+	::*'''TaST:''' Key for the CrST lock
		+	::*'''TaWk:''' Key for the CrWk lock
		+	::*'''Term:''' Terminator

---

Revision as of 22:26, 28 September 2011

Some aditional stuff.'

• Home made quick saliva DNA extraction protocol:

1. Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass
2. Prepare a solution with soap and water in a beaker
3. Add the soap solution to the last rinse
4. Add frozen (0°C) etanol to the last rinse
5. Observe how a DNA thread forms, take it and place it in an eppendorf tube.

• Abbreviations and glossary:

• Biobrickoatl:It's the biobrick deity for the Mesoamerican cultures, although at that time they didn't knew what a biobrick meant. The great feathered biobrick serpent vigilates over all new and old biobricks.
• Competent cells: Cells that can incorporate, in an easier way, foreign DNA into their cell walls.
• CcaS-EnvZ: Is the green light receptor that induces the expresión of phycobilisome linker protein.
• CFP: Cyan Fluorescent Protein
• Crst: Lock for the PbadST (strong, for low concentrations) promoter
• CrWk: Lock for the Pbad WK (weak, for high concentration) promoter
• GFP: Green Fluorescent Protein
• ho1: Heme oxigenase 1
• iTaST: Inverse TaST, complementary sequence to the Key TaST, blocks TaST activity
• pBADST: Strong arabinose promoter for low concentration detection.
• pBADWk: Weak arabinose promoter for high concentration detection.
• Pcy4A: Phycocyanobilin ferredoxin oxidoreductase.
• RBS: Ribosome binding site
• TaST: Key for the CrST lock
• TaWk: Key for the CrWk lock
• Term: Terminator