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Cell Culture
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(Created page with "'''Materials: ''' Bacterial sample LB broth 15 ml centrifuge tube '''Procedure:''' 1. Add 2ml of sterile LB broth into a 15ml centrifuge tube. 2. Inoculate the tube with 50u...")
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Revision as of 20:47, 28 September 2011
Materials: Bacterial sample
LB broth
15 ml centrifuge tube
Procedure:
1. Add 2ml of sterile LB broth into a 15ml centrifuge tube.
2. Inoculate the tube with 50ul of liquid bacterial sample or a single colony from an agar plate.
3. Incubate at 37°C from one evening to the next morning. ("overnight")
