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Team:Washington/Protocols/Cyto.
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Revision as of 19:29, 28 September 2011
Cytometry Protocol
Colonies from plates:
- Stab cells from isolated colony on plate and suspend them in 50 uL PBS buffer.
- Dilute PBS culture by adding 1 uL to 100 uL fresh PBS buffer.
- Measure fluorescence levels on cytometer.
Cells from liquid culture:
- Grow cells to mid-exponential phase (generally, OD 0.1 to 0.2).
- Dilute cells 1:20 in PBS buffer supplemented with chloramphenicol or kanamycin (choose an antibiotic the cells aren't resistant to), and store on ice.
- Measure fluorescence levels on cytometer.
