Team:USC/Project
From 2011.igem.org
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To begin, our project will utilize a synthetic version of CRISPR generated by inserting an engineered spacer that matches the DNA sequence coded within an E. coli expression plasmid. We will first test the synthetic CRISPR array against E. coli harboring a tetR regulated tetO::GFP system. We anticipate that the CRISPER tetR targeted spacer will destroy the tetR plasmid, relieving the inhibition of the tetO locus and facilitating the expression of GFP. In the near future, we hope to use the synthetic CRISPR system as a biological tool, combining it with other BioBricks and parts for use in many applications that will impact health and medicine, biotechnology, molecular biology and genetics. | To begin, our project will utilize a synthetic version of CRISPR generated by inserting an engineered spacer that matches the DNA sequence coded within an E. coli expression plasmid. We will first test the synthetic CRISPR array against E. coli harboring a tetR regulated tetO::GFP system. We anticipate that the CRISPER tetR targeted spacer will destroy the tetR plasmid, relieving the inhibition of the tetO locus and facilitating the expression of GFP. In the near future, we hope to use the synthetic CRISPR system as a biological tool, combining it with other BioBricks and parts for use in many applications that will impact health and medicine, biotechnology, molecular biology and genetics. | ||
- | == Project | + | == Project Results== |
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- | === | + | === CRISPR/cas System in K12 E. coli === |
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- | === | + | === CRISPR/cas System in B E. coli === |
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== Results == | == Results == |