Team:Queens Canada/Parts/Contributions

Materials - Thermocycler
- PCR Tubes (0.2mL)
- KAPA HiFi™ Hotstart Kit
- 10 µl of each of the 2 linear BioBrick parts to be ligated
� - 10 µM solution of left primer of BioBrick part A
- 10 µM solution of right primer of BioBrick part B
- Fermentas FastDigest SpeI system
- Fermnetas FastDigest XbaI system
- KAPA Fast Ligase system
- TBE (pH = 8.0)
- 3 µl of Ethidium Bromide
- Biotechnology grade Agarose
- Gel Box
- Power Supply
- Gel Supply
- Loading Dye
- Bio Basics EZ-10 PCR Product Purification Kit
- Transilluminator

Method

back to top

Applications

back to top

@@ Line 81: / Line 81: @@
 <div id="rightcontenttext">
 <a name="protocol"></a>
-<h3purple> Protocol </h3purple>
+<h3purple> Protocol </h3purple> <p>
+<regulartext><b>Materials </b></regulartext>
+<regulartext> - Thermocycler</regulartext><br>
+<regulartext> - PCR Tubes (0.2mL) </regulartext><br>
+<regulartext> - KAPA HiFi™ Hotstart Kit</regulartext><br>
+<regulartext> - 10 µl of each of the 2 linear BioBrick parts to be ligated</regulartext><br>
+�<regulartext> - 10 µM solution of left primer of BioBrick part A</regulartext><br>
+<regulartext> - 10 µM solution of right primer of BioBrick part B</regulartext><br>
+<regulartext> - Fermentas FastDigest SpeI system </regulartext><br>
+<regulartext> - Fermnetas FastDigest XbaI system</regulartext><br>
+<regulartext> - KAPA Fast Ligase system</regulartext><br>
+<regulartext> - TBE (pH = 8.0)</regulartext><br>
+<regulartext> - 3 µl of Ethidium Bromide</regulartext><br>
+<regulartext> - Biotechnology grade Agarose</regulartext><br>
+<regulartext> - Gel Box</regulartext><br>
+<regulartext> - Power Supply</regulartext><br>
+<regulartext> - Gel Supply</regulartext><br>
+<regulartext> - Loading Dye</regulartext><br>
+<regulartext> - Bio Basics EZ-10 PCR Product Purification Kit</regulartext><br>
+<regulartext> - Transilluminator</regulartext><p>
+<regulartext> <b> Method </b> </regulartext>