"
Team:Tec-Monterrey/projectresults
From 2011.igem.org
(Difference between revisions)
| Line 584: | Line 584: | ||
<br> | <br> | ||
| - | + | <center><img src="https://static.igem.org/mediawiki/2011/b/ba/Results01.png"> </center> | |
| + | <br> | ||
| + | <br> | ||
| + | <p class="textojustif"> There are several tecniques to prove the successful transportation of CelD and SacC on the outer membrane of <i>E. coli</i>. In this project, SDS-PAGE of entire cell culture samples, SDS-PAGE of membrane fraction samples, and measurement of enzyme activity of whole-cell-system without any chemical purification operation have been considered in order to confirm the presence of active enzymes on the external membrane of <i>E. coli</i>. | ||
<br> | <br> | ||
<br> | <br> | ||
| - | |||
| - | |||
| - | |||
<center><img src="https://static.igem.org/mediawiki/2011/9/9a/Results02.png"> </center> | <center><img src="https://static.igem.org/mediawiki/2011/9/9a/Results02.png"> </center> | ||
| + | <br> | ||
| + | <br> | ||
| + | <p class="textojustif"> | ||
| + | |||
<center><img src="https://static.igem.org/mediawiki/2011/4/40/Results03.png"> </center> | <center><img src="https://static.igem.org/mediawiki/2011/4/40/Results03.png"> </center> | ||
| + | <br> | ||
| + | <br> | ||
| + | <p class="textojustif"> | ||
| + | |||
<center><img src="https://static.igem.org/mediawiki/2011/1/1b/Results04.png"> </center> | <center><img src="https://static.igem.org/mediawiki/2011/1/1b/Results04.png"> </center> | ||
| + | <br> | ||
| + | <br> | ||
| + | <p class="textojustif"> | ||
| + | |||
<br> | <br> | ||
<br> | <br> | ||
Revision as of 13:32, 28 September 2011
There are several tecniques to prove the successful transportation of CelD and SacC on the outer membrane of E. coli. In this project, SDS-PAGE of entire cell culture samples, SDS-PAGE of membrane fraction samples, and measurement of enzyme activity of whole-cell-system without any chemical purification operation have been considered in order to confirm the presence of active enzymes on the external membrane of E. coli.
