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Team:WHU-China/Notebook
From 2011.igem.org
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<img src="/wiki/images/6/6f/Whu-Protocols.png"/> | <img src="/wiki/images/6/6f/Whu-Protocols.png"/> | ||
<img src="https://static.igem.org/mediawiki/2011/c/c6/Whu-protologo1.png" style="position:absolute;left:20px;top:40px;width:300px;height:360px;"> | <img src="https://static.igem.org/mediawiki/2011/c/c6/Whu-protologo1.png" style="position:absolute;left:20px;top:40px;width:300px;height:360px;"> | ||
| - | <div style="position:absolute;right:30px;width:620px;"> | + | <div style="position:absolute;right:30px;width:620px;font-size:15px;"> |
| - | • Dealing with biobricks | + | • Dealing with biobricks</br> |
| - | • Preparation of competent cells (Using Calcium Chloride) | + | • Preparation of competent cells (Using Calcium Chloride)</br> |
| - | • Plasmid Extraction（Using Plasmid Mini Kit） | + | • Plasmid Extraction（Using Plasmid Mini Kit）</br> |
| - | • Transformation (heat-shock method) | + | • Transformation (heat-shock method)</br> |
| - | • Cleavage with two restriction enzymes | + | • Cleavage with two restriction enzymes</br> |
| - | • Get Extraction | + | • Get Extraction</br> |
| - | • Identification of DNA by Electrophoresis | + | • Identification of DNA by Electrophoresis</br> |
| - | • Ligation | + | • Ligation</br> |
| - | • PCR of the colony | + | • PCR of the colony</br> |
| - | • Identification of DNA by Sequencing | + | • Identification of DNA by Sequencing</br> |
• Others............ | • Others............ | ||
</div> | </div> | ||
Revision as of 11:07, 28 September 2011
• Dealing with biobricks
• Preparation of competent cells (Using Calcium Chloride)
• Plasmid Extraction（Using Plasmid Mini Kit）
• Transformation (heat-shock method)
• Cleavage with two restriction enzymes
• Get Extraction
• Identification of DNA by Electrophoresis
• Ligation
• PCR of the colony
• Identification of DNA by Sequencing
• Others............
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