"
Team:WHU-China/Notebook
From 2011.igem.org
(Difference between revisions)
| Line 33: | Line 33: | ||
<img src="/wiki/images/6/6f/Whu-Protocols.png"/> | <img src="/wiki/images/6/6f/Whu-Protocols.png"/> | ||
<img src="https://static.igem.org/mediawiki/2011/c/c6/Whu-protologo1.png" style="position:absolute;left:20px;top:40px;width:300px;height:360px;"> | <img src="https://static.igem.org/mediawiki/2011/c/c6/Whu-protologo1.png" style="position:absolute;left:20px;top:40px;width:300px;height:360px;"> | ||
| + | <div style="position:absolute;right:30px;width:620px;"> | ||
| + | • Dealing with biobricks | ||
| + | • Preparation of competent cells (Using Calcium Chloride) | ||
| + | • Plasmid Extraction（Using Plasmid Mini Kit） | ||
| + | • Transformation (heat-shock method) | ||
| + | • Cleavage with two restriction enzymes | ||
| + | • Get Extraction | ||
| + | • Identification of DNA by Electrophoresis | ||
| + | • Ligation | ||
| + | • PCR of the colony | ||
| + | • Identification of DNA by Sequencing | ||
| + | • Others............ | ||
| + | </div> | ||
| + | <a href="https://2011.igem.org/Team:WHU-China/Notebook/Protocols"><img style="position:absolute;top:380px;right:100px;width:90px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a> | ||
</div> | </div> | ||
<div id="daytoday"> | <div id="daytoday"> | ||
Revision as of 11:06, 28 September 2011
• Dealing with biobricks
• Preparation of competent cells (Using Calcium Chloride)
• Plasmid Extraction（Using Plasmid Mini Kit）
• Transformation (heat-shock method)
• Cleavage with two restriction enzymes
• Get Extraction
• Identification of DNA by Electrophoresis
• Ligation
• PCR of the colony
• Identification of DNA by Sequencing
• Others............
Count Down
days
hours
minutes
seconds
Visitor
