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Team:UNAM-Genomics Mexico/Notebook/pBBR1MCS-5
From 2011.igem.org
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At beginning of the week was decided the best way to insert a prefix-RFP-suffix fragment to thepBBR1MCS-5 plasmid, which is a plasmid with broad host-range and is capable to conjugate to R. etli through E. coli S17, being the most efficient way to introduce genes into R. eltli. The pBBR1MCS-5 has a multiple cloning site (MCS) with the following restriction sites: | At beginning of the week was decided the best way to insert a prefix-RFP-suffix fragment to thepBBR1MCS-5 plasmid, which is a plasmid with broad host-range and is capable to conjugate to R. etli through E. coli S17, being the most efficient way to introduce genes into R. eltli. The pBBR1MCS-5 has a multiple cloning site (MCS) with the following restriction sites: | ||
| - | |||
KpnI | KpnI | ||
| - | |||
ApaI | ApaI | ||
XhoI | XhoI | ||
| + | |||
Sal I | Sal I | ||
| + | |||
Bsp106 I | Bsp106 I | ||
| + | |||
ClaI | ClaI | ||
| + | |||
HindIII | HindIII | ||
| + | |||
EcoR1 | EcoR1 | ||
| + | |||
PstI | PstI | ||
| + | |||
SmaI | SmaI | ||
| + | |||
Bam H1 | Bam H1 | ||
| + | |||
SpeI | SpeI | ||
| + | |||
XbaI | XbaI | ||
| + | |||
BstXI | BstXI | ||
| + | |||
SacI | SacI | ||
Revision as of 07:50, 28 September 2011
Contents |
Lab Logbook -pBBR1MCS.
Week: june 13 to 18
At beginning of the week was decided the best way to insert a prefix-RFP-suffix fragment to thepBBR1MCS-5 plasmid, which is a plasmid with broad host-range and is capable to conjugate to R. etli through E. coli S17, being the most efficient way to introduce genes into R. eltli. The pBBR1MCS-5 has a multiple cloning site (MCS) with the following restriction sites:
KpnI
ApaI
XhoI
Sal I
Bsp106 I
ClaI
HindIII
EcoR1
PstI
SmaI
Bam H1
SpeI
XbaI
BstXI
SacI
