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Team:UNAM-Genomics Mexico/Notebook/pBBR1MCS-5
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| - | + | __NOTOC__ | |
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| + | __TOC__ | ||
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| + | =Report: Adding prefix and suffix to pBBR1MCS, and characterization of it.= | ||
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| + | ==Week: june 13 to 18== | ||
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| + | At beginning of the week was decided the best way to insert a prefix-RFP-suffix fragment to thepBBR1MCS-5 plasmid, which is a plasmid with broad host-range and is capable to conjugate to R. etli through E. coli S17, being the most efficient way to introduce genes into R. eltli. The pBBR1MCS-5 has a multiple cloning site (MCS) with the following restriction sites: | ||
| + | KpnI | ||
| + | ApaI | ||
| + | XhoI | ||
| + | Sal I | ||
| + | Bsp106 I | ||
| + | ClaI | ||
| + | HindIII | ||
| + | EcoR1 | ||
| + | PstI | ||
| + | SmaI | ||
| + | Bam H1 | ||
| + | SpeI | ||
| + | XbaI | ||
| + | BstXI | ||
| + | SacI | ||
| + | |||
| + | |||
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}} | }} | ||
Revision as of 07:47, 28 September 2011
Contents |
Report: Adding prefix and suffix to pBBR1MCS, and characterization of it.
Week: june 13 to 18
At beginning of the week was decided the best way to insert a prefix-RFP-suffix fragment to thepBBR1MCS-5 plasmid, which is a plasmid with broad host-range and is capable to conjugate to R. etli through E. coli S17, being the most efficient way to introduce genes into R. eltli. The pBBR1MCS-5 has a multiple cloning site (MCS) with the following restriction sites: KpnI ApaI XhoI Sal I Bsp106 I ClaI HindIII EcoR1 PstI SmaI Bam H1 SpeI XbaI BstXI SacI
